Rat GDNF ELISA Kit (ab213901)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat GDNF ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 90pg/ml 4.32 = 4.8% 2 16 280pg/ml 22.12 = 7.9% 3 16 983pg/ml 69.79 = 7.1% Inter-assay Sample n Mean SD CV% 1 24 94pg/ml 5.54 = 5.9% 2 24 264pg/ml 24.55 = 9.3% 3 24 1071pg/ml 84.6 = 7.9% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 4 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
The Rat GDNF Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213901) is designed for the quantitative measurement of Rat GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDNF has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat GDNF amount of sample captured in plate.
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Notes
Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3, and has the ability to promote dopamine uptake in midbrain cultures. The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-rat GDNF coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Rat GDNF antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Rat GDNF standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
Tissue specificity
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
Involvement in disease
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Astrocyte derived trophic factor
- Astrocyte derived trophic factor 1
- Astrocyte-derived trophic factor
see all -
Database links
- Entrez Gene: 25453 Rat
- SwissProt: Q07731 Rat
- Unigene: 53970 Rat
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab213901 has been referenced in 6 publications.
- Wang J et al. Overexpression of microRNA-211 in Functional Dyspepsia via Downregulation of the Glial Cell Line-Derived Neurotrophic Factor (GDNF) by Increasing Phosphorylation of p38 MAPK Pathway. Can J Gastroenterol Hepatol 2022:9394381 (2022). PubMed: 36569394
- Ye K et al. In vitro study of decellularized rat tissues for nerve regeneration. Front Neurol 13:986377 (2022). PubMed: 36188412
- Wu R et al. Physical exercise promotes integration of grafted cells and functional recovery in an acute stroke rat model. Stem Cell Reports 17:276-288 (2022). PubMed: 35030322
- Li H et al. Local continuous glial cell derived neurotrophic factor release using osmotic pump promotes parasympathetic nerve rehabilitation in an animal model of cavernous nerve injury induced erectile dysfunction. Transl Androl Urol 10:258-271 (2021). PubMed: 33532315
- Hellenbrand DJ et al. Functional recovery after peripheral nerve injury via sustained growth factor delivery from mineral-coated microparticles. Neural Regen Res 16:871-877 (2021). PubMed: 33229722
- Guo J et al. Promoting potential of adipose derived stem cells on peripheral nerve regeneration. Mol Med Rep 16:7297-7304 (2017). PubMed: 28944869