• Nature
  • Amino Acid Sequence
    • Species

Associated products


Our Abpromise guarantee covers the use of ab25746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Gliomedin antibody (ab24483)

  • Form
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Cancer related gene-Liver 2
    • CLOM
    • collomin
    • COLM
    • Colm (gene name)
    • Colmedin
    • CRG L2
    • CRGL2
    • FLJ23917
    • Gldn (gene name)
    • UNC 112
    see all
  • Relevance
    Gliomedin is a member of the collagen superfamily, it is a glial ligand for neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin provides a glial cue for the formation of peripheral nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes of ranvier. Eliminating the expression of gliomedin or the addition of a soluble extracellular domain of neurofascin to myelinating cultures abolishes node formation. Gliomedin is expressed in the PNS nodes of ranvier, but not in the CNS nodes of ranvier. Gliomedin also displays high expression in murine and human hepatocellular carcinomas (HCC). Its restricted expression in normal tissues and unique early upregulation during tumor development make it an excellent candidate as a new clinical marker of HCC.
  • Cellular localization
    Cell Membrane and Cytoplasmic


ab25746 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your interesting enquiry. I have looked at the details of the two antibodies we supply to Gliomedin. As you have pointed out, to my knowledge these have not yet been tested on zebrafish. Ab24483 is a rabbit polyclonal raised against a short synthetic peptide from rat Gliomedin. Ab28675 is rabbit polyclonal raised against a short synthetic peptide from human gliomedin. The sequence from this human peptide is LVGKADEKASEHHSPQA. I hope this helps and provides you with the information you need. As you suggest, you could try doing a BLAST sequence alignment search to see how close the homology is. If you do decide to purchase this antibody, I would be very pleased to hear how you get on, especially as you will be testing it on a new species. We would be very grateful if you could submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers. If you send us an Abreview, we can offer you from 50 up to 300 Abcam points (Abpoints) based on submission time and type of application. The less characterised the product you review, the more Abpoints you will receive. Furthermore, you get 100 extra Abpoints for submitting an image. These Abpoints can then be claimed back as rewards. Good luck with your research!

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