Key features and details
- Sensitivity: 10 pg/ml
- Range: 12.35 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat GM-CSF ELISA Kit
See all GM-CSF kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 10 pg/ml
Range12.35 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 90.53 81% - 101% Serum 136.4 128% - 143% Plasma 118.8 88% - 136%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Abcam’s GM-CSF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat GM-CSF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti- rat GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-rat GM-CSF (lyophilized) 2 vials GM-CSF Microplate (12 x 8 wells) 1 unit Recombinant Rat GM-CSF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Sequence similaritiesBelongs to the GM-CSF family.
- Information by UniProt
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
ab100762 has not yet been referenced specifically in any publications.