Overview

  • Product name

    Rat GRP78 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    3 Samples 30 < 10%
    Inter-assay
    Sample n Mean SD CV%
    3 Samples 30 < 15%
  • Sample type

    Tissue Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.15 ng/ml
  • Range

    0 ng/ml - 50 ng/ml
  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s Rat GRP78 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab210604) is designed for the measurement of rat GRP78 protein in tissue lysates. The ELISA utilizes an anti-rat GRP78 monoclonal antibody-coated plate and a biotinylated antibody for detection which allows for an assay range of 0 to 50 ng/mL, with a sensitivity of 0.15 ng/mL. The other highlights of this kit are a quick incubation time, stable reagents, and an easy to use protocol.

  • Notes

    GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5’ nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed.

    GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival.

    In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress, and its protein levels are reduced in the brains of Alzheimer’s patients. Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to antitumor drugs.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    Rat GRP78 Standard 2 x 56.7ng
    Anti-GRP78 Rabbit pAb:Biotin Conjugated Detection Antibody 1 x 150µl
    Anti-Rat GRP78 Immunoassay Plate 1 x 96 tests
    Detection pAb:Biotin Diluent 1 x 13ml
    Plate Cover 2 units
    Sample and Standard Diluent Red 1 x 50ml
    Stop Solution 1 x 13ml
    Streptavidin:PolyHRP Concentrate 1 x 150µl
    Streptavidin:PolyHRP Diluent 1 x 13ml
    TMB Substrate 1 x 13ml
    Wash Buffer Concentrate (10X) 1 x 100ml
  • Research areas

  • Function

    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
  • Involvement in disease

    Autoantigen in rheumatoid arthritis.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Alternative names

    • 78 kDa glucose regulated protein
    • 78 kDa glucose-regulated protein
    • AL022860
    • AU019543
    • BIP
    • D2Wsu141e
    • D2Wsu17e
    • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
    • Endoplasmic reticulum lumenal Ca2+ binding protein grp78
    • Epididymis secretory sperm binding protein Li 89n
    • FLJ26106
    • Glucose Regulated Protein 78
    • Glucose Regulated Protein 78kDa
    • GRP 78
    • GRP-78
    • GRP78
    • GRP78_HUMAN
    • Heat shock 70 kDa protein 5
    • Heat Shock 70kDa Protein 5
    • Heat shock protein family A (Hsp70) member 5
    • HEL S 89n
    • Hsce70
    • HSPA 5
    • HSPA5
    • Immunoglobulin Heavy Chain Binding Protein
    • Immunoglobulin heavy chain-binding protein
    • mBiP
    • MIF2
    • Sez7
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab210604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard Curve Rat GRP78 ELISA kit ab210604

  • Validation of Capture Antibody:Western blot analysis of rat lysates showing the detection of ~78 kDa GRP78 protein using Anti-GRP78 Antibody, Clone 6G3-1B9, 1:1000 Dilution. MW ladder. Lane 1: Rat Liver Lysate. Lane 2: Rat Kidney Lysate; Load: 10 μg per lane. Block: 5% milk + TBST, 60min at RT. Primary antibody: Mouse Anti- GRP78 monoclonal antibody, Clone 6G3-1B9, incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody, 1:200 dilution, 60min at RT. Development: ECL solution for 6min at RT. Predicted/Observed size: ~ 78 kDa. Other band(s): ~70kDa.

Protocols

References

ab210604 has not yet been referenced specifically in any publications.

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