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Rat Haptoglobin ELISA Kit (ab108857)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (1)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 1.3 µg/ml
    • Range: 1.875 µg/ml - 120 µg/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Competitive
    • Reacts with: Rat

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    Overview

    • Product name

      Rat Haptoglobin ELISA Kit
      See all Haptoglobin kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.9%
      Inter-assay
      Sample n Mean SD CV%
      Overall 7.5%
    • Sample type

      Serum, Plasma
    • Assay type

      Competitive
    • Sensitivity

      = 1.3 µg/ml
    • Range

      1.875 µg/ml - 120 µg/ml
    • Recovery

      99 %

    • Assay time

      3h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Rat
    • Product overview

      Abcam’s Haptoglobin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.


      A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of haptoglobin captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent M Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 1 x 30ml
      4X Biotinylated Haptoglobulin (Lyophilized) 1 vial
      Chromogen Substrate 1 x 7ml
      Rat Haptoglobulin Microplate (12 x 8 well strips) 1 unit
      Rat Haptoglobulin Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 11ml
    • Research areas

      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • RBC Antigens
      • Cardiovascular
      • Blood
      • Serum Proteins
      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Cell Biology
      • Other Antibodies
      • Oxidative Stress
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cardiovascular ELISA kits
    • Function

      As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
      Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
    • Tissue specificity

      Expressed by the liver and secreted in plasma.
    • Involvement in disease

      Anhaptoglobinemia
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 peptidase S1 domain.
      Contains 2 Sushi (CCP/SCR) domains.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P00738 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Binding peptide
      • BP
      • Haptoglobin alpha chain
      • Haptoglobin alpha(1S) beta
      • Haptoglobin alpha(2FS) beta
      • Haptoglobin beta chain
      • Haptoglobin, alpha polypeptide
      • Haptoglobin, beta polypeptide
      • HP
      • HP2 ALPHA2
      • HP2ALPHA2
      • HPA1S
      • HPT
      • HPT_HUMAN
      • MGC111141
      • Zonulin
      see all
    • Database links

      • Entrez Gene: 24464 Rat
      • SwissProt: P06866 Rat
      • Unigene: 10950 Rat

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab108857.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (0)

        Publishing research using ab108857? Please let us know so that we can cite the reference in this datasheet.

        ab108857 has not yet been referenced specifically in any publications.

        Customer reviews and Q&As

        Show All Reviews Q&A
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        1-2 of 2 Abreviews or Q&A

        Determination Haptoglobin in rat milk samples

        Good Good 4/5 (Ease of Use)
        Abreviews
        Abreviews
        abreview image
        In rat milk samples, after determined different dilutions, the best dilution to fit in the standard curve is the dilution 1:250.

        Abcam user community

        Verified customer

        Submitted Jan 24 2018

        Question

        Dear technical team, Our customer would like to purchase Haptoglobin Human ELISA Kit from Abcam. I have searched it and 2 products searched. ab108858   https://www.abcam.com/Haptoglobulin-Human-ELISA-Kit-ab108858.html ab108856  https://www.abcam.com/Haptoglobin-Human-ELISA-Kit-ab108856.html Please let me know the difference Haptoglobulin and Haptoglobin. Because our customer said there's no Haptoglobulin as far as he know so, please confirm this. Best regards,

        Read More

        Abcam community

        Verified customer

        Asked on Dec 20 2011

        Answer

        Thank you for contacting us and sorry for the delay in getting back to you. Your customer is correct in thinking that the Haptoglobin and Haptoglobulin refer to the same protein. I am sorry that our datasheets have caused confusion. I will be looking into remedying this to use a uniform name for the products. I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

        Read More

        Abcam Scientific Support

        Answered on Dec 20 2011

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

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