Rat heart tissue lysate - mitochondrial extract (contains SDS/DTT) (ab110341)

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Overview

  • Product name

    Rat heart tissue lysate - mitochondrial extract (contains SDS/DTT)
    See all Rat Heart lysates

  • Description

    Rat Heart Mitochondrial lysate

  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    50 µg of rat heart mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

  • Tested applications

    Suitable for: SDS-PAGE, WBmore details

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.5
    Constituent: 99% SDS-PAGE Sample Buffer
  • Concentration information loading...

  • Lysate notes

    50 µg of rat heart mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution. 10.0 µg/lane
Notes
SDS-PAGE
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution. 10.0 µg/lane

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab110341? Please let us know so that we can cite the reference in this datasheet.

ab110341 has been referenced in 1 publication.

  • Frapin M  et al. Maternal Protein Restriction in Rats Alters the Expression of Genes Involved in Mitochondrial Metabolism and Epitranscriptomics in Fetal Hypothalamus. Nutrients 12:N/A (2020). PubMed: 32438566

Customer reviews and Q&As

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Question

vielen, vielen Dank für Ihr Angebot uns die Kontrolle zuzusenden. Schicken Sie diese so wie Sie vorgeschlagen haben an die Zentralapotheke.

Mit freundlichen Grüßen

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Answer

Vielen Dank für Ihre Antwort.

Wie besprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben mit der positiven Kontrolle ab110341. https://www.abcam.com/Rat-heart-tissue-lysate-mitochondrial-extract-contains-SDSDTT-ab110341.html (or use the following: https://www.abcam.com/Rat-heart-tissue-lysate-mitochondrial-extract-contains-SDSDTT-ab110341.html).

Die Ersatzlieferunghat die Referenznummer xx und sollte morgen bei Ihnen ankommen.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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