Overview

  • Product name
    Rat Hemoglobin ELISA Kit
    See all Hemoglobin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma, Other biological fluids
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.428 ng/ml
  • Range
    0.006 µg/ml - 16.92 µg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam's Hemoglobin Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemoglobin in biological samples of rats.

    In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hemoglobin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Hemoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemoglobin in the test sample. The quantity ofHemoglobinin the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-rat Hemoglobin antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Rat Hemoglobin Calibrator (Lyophilized) 1 vial
    Rat Hemoglobin ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Involved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificity
    Red blood cells.
  • Involvement in disease
    Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
    Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
    Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
  • Sequence similarities
    Belongs to the globin family.
  • Post-translational
    modifications
    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • Alternative names
    • 3-prime alpha-globin gene
    • Alpha 2 globin chain
    • Alpha globin
    • alpha one globin
    • alpha-1 globin
    • Alpha-globin
    • Beta globin
    • CD113t C
    • CD31
    • Delta globin
    • Erythremia, beta-globin type, included
    • Gamma 1 globin
    • Hb F Agamma
    • HBA
    • HBA 1
    • HBA 2
    • HBA T3
    • HBA-T2
    • HBA_HUMAN
    • HBA1
    • HBA2
    • HBB
    • Hbb-y
    • HBD
    • Hbe1
    • HBG
    • HBG 1
    • HBG1
    • HBGA
    • HBGR
    • HBH
    • Hemoglobin alpha 1
    • hemoglobin alpha 1 globin chain
    • Hemoglobin alpha chain
    • Hemoglobin alpha locus
    • Hemoglobin alpha locus 1
    • hemoglobin alpha-1 chain
    • Hemoglobin beta
    • Hemoglobin beta chain
    • Hemoglobin beta chain complex
    • Hemoglobin beta locus
    • Hemoglobin gamma
    • Hemoglobin gamma 1 chain
    • Hemoglobin gamma A
    • Hemoglobin gamma A chain
    • Hemoglobin subunit alpha
    • Hemoglobin subunit beta
    • Hemoglobin subunit gamma 1
    • hemoglobin, gamma, regulator of
    • Hemoglobin--gamma locus, 136 alanaine
    • HSGGL1
    • LVV-hemorphin-7
    • Methemoglobinemia, beta-globin type, included
    • MGC126895
    • MGC126897
    • Minor alpha-globin locus
    • PRO2979
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab157733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157733 Hemoglobin Rat ELISA Kit.

Protocols

References

ab157733 has not yet been referenced specifically in any publications.

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