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    rat-hemopexin-elisa-kit-hpx-ab157734.pdf

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Rat Hemopexin ELISA Kit (HPX) (ab157734)

  • Datasheet
  • SDS
  • Protocol Booklet
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Standard Curve

    Key features and details

    • Sensitivity: 1.1 ng/ml
    • Range: 6.25 ng/ml - 400 ng/ml
    • Sample type: Other biological fluids, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

    You may also be interested in

    Protein
    Product image
    Native Rat Hemopexin protein (ab198629)

    View more associated products

    Overview

    • Product name

      Rat Hemopexin ELISA Kit (HPX)
      See all Hemopexin kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%
    • Sample type

      Serum, Plasma, Other biological fluids
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      1.1 ng/ml
    • Range

      6.25 ng/ml - 400 ng/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum > 85 % - %
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Rat
    • Product overview

      Abcam's Hemopexin (HPX) Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hemopexin (HX) in biological samples of rats.


      In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-HX antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound HX. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of HX in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of HX in the test sample. The quantity of HX in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


      ab157734 has been redeveloped with new goat capture and detector antibodies, Kits purchased prior to 2020 used chicken antibodies.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X HRP-conjugated anti-rat Hemopexin antibody 1 x 150µl
      20X Wash Buffer Concentrate 1 x 50ml
      5X Diluent Concentrate 1 x 50ml
      Chromogen Substrate Solution 1 x 12ml
      Rat Hemopexin Calibrator (lyophilized) 1 vial
      Rat IgA ELISA Microplate 1 x 12 units
      Stop Solution 1 x 12ml
    • Research areas

      • Cardiovascular
      • Blood
      • Other
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP
      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Signal transduction proteins ELISA kits
      • Metabolism
      • Pathways and Processes
      • Cofactors, Vitamins / minerals
      • Vitamins / minerals
    • Function

      Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
    • Tissue specificity

      Expressed by the liver and secreted in plasma.
    • Sequence similarities

      Belongs to the hemopexin family.
      Contains 5 hemopexin-like domains.
    • Post-translational
      modifications

      N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02790 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Beta 1B glycoprotein
      • Beta-1B-glycoprotein
      • FLJ56652
      • HEMO
      • HEMO_HUMAN
      • Hemopexin
      • Hpx
      • HX
      see all
    • Database links

      • Entrez Gene: 58917 Rat
      • SwissProt: P20059 Rat
      • Unigene: 2380 Rat

      Associated products

        Images

        • Standard Curve
          Standard Curve
          Representative standard curve using ab157734 Hemopexin Rat ELISA Kit.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (1)

        Publishing research using ab157734? Please let us know so that we can cite the reference in this datasheet.

        ab157734 has been referenced in 1 publication.

        • Paul S  et al. STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox Biol 14:423-438 (2018). ELISA ; Rat . PubMed: 29078168

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