Rat ICAM1 ELISA Kit (CD54) (ab100763)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Rat ICAM1 ELISA Kit (CD54)
See all ICAM1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
28.81 pg/ml - 7000 pg/ml -
Recovery
91 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.54 89% - 107% Serum 89.42 80% - 101% Plasma 85.54 76% - 95% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s ICAM1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat ICAM- 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Rat ICAM1 coated on a 96- well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Rat ICAM1 2 vials ICAM1 Microplate (12 x 8 wells) 1 unit Recombinant Rat ICAM1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria. -
Cellular localization
Membrane -
Alternative names
- BB2
- CD 54
- CD54
see all -
Database links
- Entrez Gene: 25464 Rat
- SwissProt: Q00238 Rat
- Unigene: 12 Rat
Images
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Representative Standard CurveRepresentative Standard Curve using ab100763
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Representative Standard CurveRepresentative Standard Curve using ab100763
Protocols
References
This product has been referenced in:
- Fahmi AA et al. Chemical composition and protective role of Pulicaria undulata (L.) C.A. Mey. subsp. undulata against gastric ulcer induced by ethanol in rats. Heliyon 5:e01359 (2019). Read more (PubMed: 30957042) »
- Fahmi AA et al. Pulicaria crispa mitigates gastric ulcer induced by ethanol in rats: Role of treatment and auto healing. Biomarkers N/A:1-32 (2018). Read more (PubMed: 30512969) »
