Rat ICAM1 ELISA Kit (CD54) (ab100763)

Submit a review Q&A (1)References (6)
Representative Standard Curve
  • Representative Standard Curve

Key features and details

  • Sensitivity: 20 pg/ml
  • Range: 28.81 pg/ml - 7000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat ICAM1 ELISA Kit (CD54)
    See all ICAM1 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 20 pg/ml

  • Range

    28.81 pg/ml - 7000 pg/ml

  • Recovery

    91 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.54 89% - 107%
    Serum 89.42 80% - 101%
    Plasma 85.54 76% - 95%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Abcam’s ICAM1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat ICAM- 1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Rat ICAM1 coated on a 96- well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Rat ICAM1 2 vials
    ICAM1 Microplate (12 x 8 wells) 1 unit
    Recombinant Rat ICAM1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Relevance

    ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

  • Cellular localization

    Membrane

  • Alternative names

    • BB2
    • CD 54
    • CD54
    • CD54 antigen
    • Cell surface glycoprotein P3.58
    • Human rhinovirus receptor
    • ICAM 1
    • ICAM-1
    • Intercellular adhesion molecule 1
    • Major group rhinovirus receptor
    • MALA2
    • MGC6195
    • MyD10
    • P3.58
    • sICAM-1
    • Surface antigen of activated B cells
    see all

  • Database links

    Images

    • Representative Standard Curve
      Representative Standard Curve

      Representative Standard Curve using ab100763

    • Representative Standard Curve
      Representative Standard Curve

      Representative Standard Curve using ab100763

    References (6)

    Publishing research using ab100763? Please let us know so that we can cite the reference in this datasheet.

    ab100763 has been referenced in 6 publications.

    • Fahmi AA  et al. Chemical composition and protective role of Pulicaria undulata (L.) C.A. Mey. subsp. undulata against gastric ulcer induced by ethanol in rats. Heliyon 5:e01359 (2019). PubMed: 30957042
    • Zhao C  et al. Up-regulation of ANXA1 suppresses polymorphonuclear neutrophil infiltration and myeloperoxidase activity by activating STAT3 signaling pathway in rat models of myocardial ischemia-reperfusion injury. Cell Signal 62:109325 (2019). PubMed: 31132398
    • Fahmi AA  et al. Pulicaria crispa mitigates gastric ulcer induced by ethanol in rats: Role of treatment and auto healing. Biomarkers N/A:1-32 (2018). PubMed: 30512969
    • Liu WY  et al. Zerumbone, a Bioactive Sesquiterpene, Ameliorates Diabetes-Induced Retinal Microvascular Damage through Inhibition of Phospho-p38 Mitogen-Activated Protein Kinase and Nuclear Factor-?B Pathways. Molecules 21:N/A (2016). PubMed: 27973425
    • Xie TY  et al. Effect of ozone on vascular endothelial growth factor (VEGF) and related inflammatory cytokines in rats with diabetic retinopathy. Genet Mol Res 15:N/A (2016). PubMed: 27323014
    • Tzeng TF  et al. Consumption of Polyphenol-Rich Zingiber Zerumbet Rhizome Extracts Protects against the Breakdown of the Blood-Retinal Barrier and Retinal Inflammation Induced by Diabetes. Nutrients 7:7821-41 (2015). PubMed: 26389948

    Customer reviews and Q&As

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    Question

    We have received an ICAM1 Rat ELISA kit (ab100763) and the Instructions for use do not indicate the anticoagulant used for blood collection.

    Can you indicate us the better anticoagulant to use?

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    Answer

    EDTA, Heparin, or Citrate collected plasma can be used with this kit. Other anti-coagulants are not necessarily excluded but have not been tested.

    Read More

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