Rat IgE ELISA Kit (ab157736)

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Sample
  • Standard curve

Key features and details

  • Sensitivity: 1.4192 ng/ml
  • Range: 1 ng/ml - 32 ng/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat IgE ELISA Kit
    See all IgE kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%

  • Sample type

    Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.4192 ng/ml

  • Range

    1 ng/ml - 32 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Abcam’s IgE Rat ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgE in rat samples.

    In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-rat IgE antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Lyophilized Rat IgE Calibrator 1 vial
    Rat IgE ELISA Microplate 1 unit
    Stop Solution 1 x 12ml

  • Research areas

  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.

  • Cellular localization

    Secreted

  • Alternative names

    • Ig epsilon chain C region
    • IgE
    • IGHE
    • immunoglobulin heavy constant epsilon

  • Database links

    Associated products

      Images

      • Sample
        Sample

        IgE measured in biological fluids showing quantity (ng) per mL of tested sample

      • Standard curve
        Standard curve

        Standard curve: mean of duplicates (+/- SD) with background reads subtracted.

      References (7)

      Publishing research using ab157736? Please let us know so that we can cite the reference in this datasheet.

      ab157736 has been referenced in 7 publications.

      • Lenz KM  et al. Prenatal Allergen Exposure Perturbs Sexual Differentiation and Programs Lifelong Changes in Adult Social and Sexual Behavior. Sci Rep 9:4837 (2019). PubMed: 30886382
      • Li X  et al. The Protective Effect of a Topical Mucin Secretagogue on Ocular Surface Damage Induced by Airborne Carbon Black Exposure. Invest Ophthalmol Vis Sci 60:255-264 (2019). PubMed: 30649152
      • Eftekhar N  et al. Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma. BMC Complement Altern Med 19:349 (2019). PubMed: 31801507
      • Zhang XY  et al. GAS5 promotes airway smooth muscle cell proliferation in asthma via controlling miR-10a/BDNF signaling pathway. Life Sci 212:93-101 (2018). PubMed: 30189218
      • Wills PJ  et al. Population Explosions of Tiger Moth Lead to Lepidopterism Mimicking Infectious Fever Outbreaks. PLoS One 11:e0152787 (2016). PubMed: 27073878
      • Eom Y  et al. The Effect of Ambient Titanium Dioxide Microparticle Exposure to the Ocular Surface on the Expression of Inflammatory Cytokines in the Eye and Cervical Lymph Nodes. Invest Ophthalmol Vis Sci 57:6580-6590 (2016). PubMed: 27918832
      • Seiffert J  et al. Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726 (2015). Rat . PubMed: 25747867

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