Rat IgE ELISA Kit (ab157736)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameRat IgE ELISA Kit
See all IgE kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample typeSerum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity1.419 ng/ml
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Range1 ng/ml - 32 ng/ml
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Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Rat
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Product overview
Abcam’s IgE Rat ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgE in rat samples.
In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 100X HRP-conjugated anti-rat IgE antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Lyophilized Rat IgE Calibrator 1 vial Rat IgE ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
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Cellular localizationSecreted
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Alternative names
- Ig epsilon chain C region
- IgE
- IGHE
- immunoglobulin heavy constant epsilon
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Database links
- Entrez Gene: 299351 Rat
- SwissProt: P01855 Rat
Applications
Our Abpromise guarantee covers the use of ab157736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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SampleIgE measured in biological fluids showing quantity (ng) per mL of tested sample
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Standard curveStandard curve: mean of duplicates (+/- SD) with background reads subtracted.
Protocols
References
This product has been referenced in:
- Seiffert J et al. Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726 (2015). Rat . Read more (PubMed: 25747867) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"