Key features and details
- Sensitivity: 4 ng/ml
- Range: 16 ng/ml - 2000 ng/ml
- Sample type: Ascites Fluid, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Quantitative
Product nameRat IgG ELISA kit - 30 minutes
See all IgG kits
Intra-assay Sample n Mean SD CV% A 1/10 10 5.18µg/ml 2.94 % B 1/10 10 2.39µg/ml 3.48 % C 1/10 10 1.34µg/ml 4.61 % Inter-assay Sample n Mean SD CV% D 1/100 30 3.2 % D 1/400 30 3.59 % D 1/6400 30 3.13 %
Sample typeCell culture supernatant, Ascites Fluid
Range16 ng/ml - 2000 ng/ml
Assay durationOne step assay
The Rat IgG ELISA Kit (ab273153) provides a rapid and easy method (one step ELISA) for the quantitative determination of rat IgG in cell culture supernatant and rat ascitic fluid.
A polyclonal antibody specific to rat IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and rat IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-rat IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of rat IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10 x 96 tests Detection antibody 1 x 12ml 1 x 120ml Pre-coated microwells strips 1 x 96 tests 10 x 96 tests Rat IgG standard 0.0 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 1000 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 125 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 16 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 2000 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 250 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 500 ng/ml 1 x 0.3ml 1 x 1ml Sample Diluent 1 x 30ml 1 x 500ml Stop solution 1 x 12ml 1 x 120ml TMB Substrate 1 x 12ml 1 x 120ml
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
ab273153 has not yet been referenced specifically in any publications.