- Datasheet
- Specific References
- Protocols
Overview
-
Product nameRat IgG ELISA Kit
See all IgG kits -
Detection methodColorimetric
-
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample typeSerum, Plasma
-
Assay typeSandwich (quantitative)
-
Sensitivity4.535 ng/ml
-
Range25 ng/ml - 800 ng/ml
-
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay durationMultiple steps standard assay
-
Species reactivityReacts with: Rat
-
Product overview
Abcam’s IgG Rat ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in rat serum and plasma samples.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get results in 90 minutes with Rat IgG ELISA Kit (ab189578) from our SimpleStep ELISA® range.
-
Tested applicationsSuitable for: Sandwich ELISAmore details
-
PlatformMicroplate
Properties
-
Storage instructionsPlease refer to protocols.
-
Components 1 x 96 tests 100X HRP-conjugated anti-rat IgG antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat IgG Calibrator 1 vial Rat IgG ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
-
RelevanceImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
-
Cellular localizationCell Membrane and Secreted
-
Alternative names
- Ig gamma
- Ig gamma-1 chain C region
- IGHG1
- Immunoglobulin G
-
Database links
- Entrez Gene: 299354 Rat
- SwissProt: P20759 Rat
Associated products
-
SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab157737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
-
Sandwich ELISA - ab157737 IgG Rat ELISA KitStandard curve: mean of duplicates (+/- SD) with background readings subtracted
-
Sandwich ELISA - ab157737 IgG Rat ELISA KitRat IgG measured in biofluids showing quantity (micrograms) per mL of tested sample
-
Sandwich ELISA - ab157737 IgG Rat ELISA KitRat IgG measured in biologicals at various dilutions showing quantity (ng) per mL of tested sample
Protocols
References
ab157737 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"