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Immunology Immunoglobulins Heavy Chain IgG
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Rat IgG ELISA Kit (ab157737)

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  • SDS
  • Protocol Booklet
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Sandwich ELISA - ab157737 IgG Rat ELISA Kit
  • Sandwich ELISA - ab157737 IgG Rat ELISA Kit
  • Sandwich ELISA - ab157737 IgG Rat ELISA Kit

Key features and details

  • Sensitivity: 4.5349 ng/ml
  • Range: 25 ng/ml - 800 ng/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

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Overview

  • Product name

    Rat IgG ELISA Kit
    See all IgG kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.5349 ng/ml
  • Range

    25 ng/ml - 800 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s IgG Rat ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in rat serum and plasma samples.


    In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


    Get results in 90 minutes with Rat IgG ELISA Kit (ab189578) from our SimpleStep ELISA® range.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-rat IgG antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Rat IgG Calibrator 1 vial
    Rat IgG ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Immunoglobulins ELISA kits
  • Cellular localization

    Secreted
  • Alternative names

    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Associated products

  • SimpleStep ELISA kits

    • Rat IgG ELISA Kit (ab189578)

Images

  • Sandwich ELISA - ab157737 IgG Rat ELISA Kit
    Sandwich ELISA - ab157737 IgG Rat ELISA Kit

    Standard curve: mean of duplicates (+/- SD) with background readings subtracted

  • Sandwich ELISA - ab157737 IgG Rat ELISA Kit
    Sandwich ELISA - ab157737 IgG Rat ELISA Kit

    Rat IgG measured in biofluids showing quantity (micrograms) per mL of tested sample

  • Sandwich ELISA - ab157737 IgG Rat ELISA Kit
    Sandwich ELISA - ab157737 IgG Rat ELISA Kit

    Rat IgG measured in biologicals at various dilutions showing quantity (ng) per mL of tested sample

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab157737? Please let us know so that we can cite the reference in this datasheet.

ab157737 has not yet been referenced specifically in any publications.

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