Rat IgG ELISA Kit (ab157737)
- Datasheet
- References
- Protocols
Overview
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Product name
Rat IgG ELISA Kit
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.535 ng/ml -
Range
25 ng/ml - 800 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s IgG Rat ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in rat serum and plasma samples.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get results in 90 minutes with Rat IgG ELISA Kit (ab189578) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-rat IgG antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat IgG Calibrator 1 vial Rat IgG ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
Associated products
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SimpleStep ELISA kits
Images
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Sandwich ELISA - ab157737 IgG Rat ELISA KitStandard curve: mean of duplicates (+/- SD) with background readings subtracted
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Sandwich ELISA - ab157737 IgG Rat ELISA KitRat IgG measured in biofluids showing quantity (micrograms) per mL of tested sample
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Sandwich ELISA - ab157737 IgG Rat ELISA KitRat IgG measured in biologicals at various dilutions showing quantity (ng) per mL of tested sample
Protocols
References
ab157737 has not yet been referenced specifically in any publications.
