Rat IgG - Isotype Control (ab37361)

Q&A (8)References (5)

Overview

  • Product name

    Rat IgG - Isotype Control
    See all Rat isotype controls

  • Specificity

    This antibody has been selected to be an isotype control as it has no known specificity.

  • Tested applications

    Suitable for: Flow Cyt, ELISAmore details

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

  • Alternative names

    • Rat Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Notes
Flow Cyt
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.

References (5)

Publishing research using ab37361? Please let us know so that we can cite the reference in this datasheet.

ab37361 has been referenced in 5 publications.

  • Koike K  et al. Subcutaneous administration of neutralizing antibodies to endothelial monocyte-activating protein II attenuates cigarette smoke-induced lung injury in mice. Am J Physiol Lung Cell Mol Physiol 316:L558-L566 (2019). PubMed: 30628489
  • Fest S  et al. Targeting of heme oxygenase-1 as a novel immune regulator of neuroblastoma. Int J Cancer 138:2030-42 (2016). PubMed: 26595750
  • Cao Y  et al. BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity. Cancer Res 74:5091-102 (2014). IHC . PubMed: 25224959
  • Postupna NO  et al. Flow cytometry analysis of synaptosomes from post-mortem human brain reveals changes specific to Lewy body and Alzheimer's disease. Lab Invest 94:1161-72 (2014). Flow Cyt . PubMed: 25068655
  • Wick N  et al. Lymphatic precollectors contain a novel, specialized subpopulation of podoplanin low, CCL27-expressing lymphatic endothelial cells. Am J Pathol 173:1202-9 (2008). PubMed: 18772332

Customer reviews and Q&As

Q&A
Submit a question

1-8 of 8 Q&A

Question

Inquiry: is ab37361 endotoxin tested? we would like to inject the ab into mice as a control for experimental Ab.

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Answer



I can confirm that ab37361 is not tested to be endotoxin free and therefore is most probably not suitable for your application.

But I am happy to point you to ab37371 (Rat IgG (low endotoxin, azide free) - Isotype Control) which is tested to be low endotoxin (Endotoxin level of <25EU/mL) and azide free:

https://www.abcam.com/Rat-IgG-Isotype-Control-Low-endotoxin-Azide-free-ab37371.html (or use the following: https://www.abcam.com/Rat-IgG-Isotype-Control-Low-endotoxin-Azide-free-ab37371.html).

ab37371 is not tested for in vivo applications and therefore is not covered by our guarantee.

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Question

Dear Supplier,



Please see customers request below:

Kindly let me know if the Rat IgG abcam ab37361 is specific to Ig2b. We need the antibody as a control. We will perform IP injections to mice and check to see depletion for some other antibodies. The rat IgG2b antibody will be used as a control.



Please let me know if you require any further information.





Thanks





Kind regards,

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Answer

Thank you for your inquiry.

I am sorry to confirm that ab37361 is not aa IgG2b antibody but a mixture of rat IgGs of all isotypes.

I can suggest to following antibody for your customer's experiments:

ab18536 (Rat IgG2b, kappa monoclonal [RTK4530] - BSA/Azide free)

It is of the IgG2b isotype and is supplied in PBS. Please review the datasheet for more information:

https://www.abcam.com/Rat-IgG2b-kappa-monoclonal-RTK4530-Isotype-Control-BSA-and-Azide-free-ab18536.html (or use the following: https://www.abcam.com/Rat-IgG2b-kappa-monoclonal-RTK4530-Isotype-Control-BSA-and-Azide-free-ab18536.html).

I hope this information is helpful and wish you a good mid week :-)

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Question

Product code: 37361
Lot number: GR82732-1
Inquiry: Hi there, I am looking for a certificate of analysis for the above product and lot number. If you could send this to me asap I would be very grateful. Many Thanks,

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Answer

Thank you for your enquiry regarding ab37361.

Please find enclosed the requested CoC as attached word document.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Product code: 37361
Inquiry: Can you tell me please as are purified immunoglobulins from normal rat sera, protein A, G others? sincerely

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Answer

Thank you for your enquiry.

I can confirm that ab37361 is IgG fraction. There are several different purity of isotypes and secondary antibodies are available. You can conduct a search using our advanced search engine and select the requested purity.

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

https://www.abcam.com/index.html?c=718

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Hello,

Yes I would be interested by your offer.
I already ask for a quote (see below) to orders@abcam.com
Thanks,

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Answer

Thank you for your reply. I am very pleased to hear you would like to accept our offer and test ab37361 and ab37355 in ChIP.

Thank you also for requesting the proforma invoice. We do not have smaller sizes of these antibody controls. The proforma my colleague sent you, is the smallest size we have.

Please find here the discount codes:
xxxx


I am very pleased to hear you would like to accept our offer and test ab37361 and ab37355 in ChIP.Each of thesecodes will give you: 1 free antibody order before the expiration date. To redeem this offer, please submit an Abreview forChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

Yes, I would like to test them in ChIP experiment.
Would it be possible to have a quote for one IgG mouse and one IgG rat?

Thank you in advance,

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Answer

Thank you very much for your interest in our mouse and rat IgG. The offer consists actually in a 100% discount on a second purchase : please see here for the details:

As to our knowledge,the mouse IgG fraction ab37355 and the rat IgG fraction ab37361 havenot yetbeen tested in ChIP. Therefore, I can offer a discount off a future purchase if you buy ab37361 and/or ab37355 now, test it inChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of:1 freeprimary antibodyfor each antibody tested- so2 free antibodies ifyou test both, ab37361 and ab37355.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab37361 and/or ab37355 in ChIP. I will then send a discount code. This code must be issued before purchasing ab37361 and ab37355 so please wait for my reply before ordering.

2. Purchase ab37361 and ab37355 either by phone, fax, or online (www.abcam.com).

3. Test it in ChIP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab37361 and ab37355 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question

Which IgG isotype is ab78287?
Is there an isotype control available, tested in IHC-P and IHC-Fr?

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Answer

Thank you for your recent telephone enquiry.

I am sorry to confirm that the IgG isotype subtype of ab78287 has not been determined.

I can suggest you may like to consider trying the following rat IgG isotype control. However, please note this has not been tested and guaranteed in IHC-P and IHC-Fr.

ab37361Rat IgG - Isotype Control
Tested in: Flow Cyt, ELISA
https://www.abcam.com/Rat-IgG-Isotype-Control-ab37361.html (or use the following: https://www.abcam.com/Rat-IgG-Isotype-Control-ab37361.html).

Also, here is a link to our isotype control webpage, for future reference:
https://www.abcam.com/index.html?c=718

I am sorry we have no suitably tested products to suggest on this occasion, but I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

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Question

Good day! We have a customer here with a technical enquiry about the above antibody. They would like to know about the specific isotype of this antibody. I hope to hear from you soon.

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Answer

Thank you for your patience. This product is a polyclonal Rat IgG from normal Rat sera. Unfortunately, the isotype had not been determined. However, it should exhibit the normal distribution of IgG sub-isotypes found in normal sera. I am sorry that we do not have more information to provide regarding this product. If there is anything else that I can help you with, please do not hesitate to contact me.

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