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    rat-igg1-kappa-monoclonal-rtk2071-isotype-control-ab18412.pdf

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Isotype/Loading Controls Isotype Controls Rat
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Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

  • Datasheet
  • SDS
Q&A (3)References (1)

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Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

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    Overview

    • Product name

      Rat IgG1, kappa monoclonal [RTK2071] - Isotype control
      See all Rat isotype controls
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

    • General notes


      The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Isotype control notes

      The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
    • Clonality

      Monoclonal
    • Clone number

      RTK2071
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Isotype/Loading Controls
      • Isotype Controls
      • Rat
    • Alternative names

      • Rat Isotype Control

    Associated products

    • Alternative Versions

      • Biotin Rat IgG1, kappa [RTK2071] - isotype control (ab18403)
      • FITC Rat IgG1, kappa [RTK2071] - isotype control (ab18404)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18412 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 1µg for 106 cells.
    Notes
    Flow Cyt
    Use 1µg for 106 cells.

    Images

    • Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
      Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

      Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab18412? Please let us know so that we can cite the reference in this datasheet.

    ab18412 has been referenced in 1 publication.

    • Monfared MH  et al. Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells. Iran J Basic Med Sci 19:1186-1192 (2016). PubMed: 27917274

    Customer reviews and Q&As

    Q&A
    Submit a question

    1-3 of 3 Q&A

    Question

    Customer kindly contacted us regarding the availability of an Igg1 isotype control for ab13789

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2012

    Answer


    Our Rat IgG1, kappa monoclonal Isotype control is a suitable isotype control for ab13789 and has been tested and guaranteed to work in IHC-frozen. A link for this isotype control is given below:
    ab18412 (https://www.abcam.com/Rat-IgG1-kappa-monoclonal-RTK2071-Isotype-control-ab18412.html)

    Read More

    Abcam Scientific Support

    Answered on Dec 07 2012

    Question

    MSDS needed for
    ab3104
    ab18412
    ab45139
    ab68672

    Read More

    Abcam community

    Verified customer

    Asked on Aug 09 2012

    Answer

    Please find attached to this email the MSDS you requested for the following antibodies:
    ab3104
    ab18412
    ab45139
    ab68672

    A link to each of those MSDS will be shortly available from the product datasheets. This usually takes a few hours up to one day, delay that is necessary for the cache of the website to be regenerated.

    I hope these documents are useful to you. Do not hesitate to contact us again if you have further questions.

    I wish you good luck with your research work.

    Read More

    Abcam Scientific Support

    Answered on Aug 09 2012

    Question

    Dear,

    I put in this email all the reference for which I need a MSDS.

    ab45139
    ab3104
    ab68672
    ab45139
    ab18412

    Thanks in advance for your answer,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 07 2012

    Answer

    Thank you for contacting us.

    I would like to inform you that we are currently dealing with your request.

    I will contact you again as soon as the requested documents are available.

    Thank you for your patience.

    Read More

    Abcam Scientific Support

    Answered on Aug 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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