Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameRat IgG1, kappa monoclonal [RTK2071] - Isotype control
See all Rat isotype controls -
Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cyt, WB, IPmore details
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Immunogen
Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.
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General notes
The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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Isotype control notesThe ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
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ClonalityMonoclonal
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Clone numberRTK2071
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IsotypeIgG1
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Light chain typekappa
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Research areas
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Alternative names
- Rat Isotype Control
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab18412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| Flow Cyt | Use 2µg for 106 cells. ab18412 is a negative isotype control and can be used for surface or intracellular staining | |
| WB | Use at an assay dependent dilution. | |
| IP | Use at an assay dependent dilution. |
Images
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![Flow Cytometry - Anti-GTPase HRAS antibody [Y13-259] (ab16881)](https://www.abcam.com/ps/datasheet/images/16/ab16881/GTPase-HRAS-Primary-antibodies-ab16881-1.jpg)
Flow Cytometry - Anti-GTPase HRAS antibody [Y13-259] (ab16881)Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
References
This product has been referenced in:
- Monfared MH et al. Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells. Iran J Basic Med Sci 19:1186-1192 (2016). Read more (PubMed: 27917274) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"