Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

Q&A (3)References (1)

Overview

  • Product name
    Rat IgG1, kappa monoclonal [RTK2071] - Isotype control
    See all Rat isotype controls
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cyt, WB, IPmore details
  • Immunogen

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • General notes


    The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Isotype control notes
    The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality
    Monoclonal
  • Clone number
    RTK2071
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 2µg for 106 cells. ab18412 is a negative isotype control and can be used for surface or intracellular staining
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Images

  • Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
    Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

    Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:
  • Monfared MH  et al. Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells. Iran J Basic Med Sci 19:1186-1192 (2016). Read more (PubMed: 27917274) »
See 1 Publication for this product

Publishing research using ab18412? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Q&A
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1-3 of 3 Q&A

Question

Customer kindly contacted us regarding the availability of an Igg1 isotype control for ab13789

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Answer


Our Rat IgG1, kappa monoclonal Isotype control is a suitable isotype control for ab13789 and has been tested and guaranteed to work in IHC-frozen. A link for this isotype control is given below:
ab18412 (https://www.abcam.com/Rat-IgG1-kappa-monoclonal-RTK2071-Isotype-control-ab18412.html)

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Question

MSDS needed for
ab3104
ab18412
ab45139
ab68672

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Answer

Please find attached to this email the MSDS you requested for the following antibodies:
ab3104
ab18412
ab45139
ab68672

A link to each of those MSDS will be shortly available from the product datasheets. This usually takes a few hours up to one day, delay that is necessary for the cache of the website to be regenerated.

I hope these documents are useful to you. Do not hesitate to contact us again if you have further questions.

I wish you good luck with your research work.

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Question

Dear,

I put in this email all the reference for which I need a MSDS.

ab45139
ab3104
ab68672
ab45139
ab18412

Thanks in advance for your answer,

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Answer

Thank you for contacting us.

I would like to inform you that we are currently dealing with your request.

I will contact you again as soon as the requested documents are available.

Thank you for your patience.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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