Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
![Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)](https://www.abcam.com/ps/products/18/ab18412/Images/ab18412-28037-rat-igg1-kappa-monoclonal-rtk2071-isotype-control-flow-cytometry.jpg "Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)")
Overview
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Product name
Rat IgG1, kappa monoclonal [RTK2071] - Isotype control
See all Rat isotype controls -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.
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General notes
The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Isotype control notes
The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. -
Clonality
Monoclonal -
Clone number
RTK2071 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
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Alternative names
- Rat Isotype Control
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
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| Notes |
|---|
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Flow Cyt
Use 1µg for 106 cells. |
Images
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Flow Cytometry - Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
References (1)
ab18412 has been referenced in 1 publication.
- Monfared MH et al. Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells. Iran J Basic Med Sci 19:1186-1192 (2016). PubMed: 27917274
