Rat IgG1, kappa [RTK2071] (biotin) - isotype control (ab18403)

Overview

  • Product name
    Rat IgG1, kappa [RTK2071] (biotin) - isotype control
    See all Rat isotype controls
  • Conjugation
    Biotin
  • Conjugation notes
    The antibody was conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
  • Specificity
    The RTK2071 immunoglobulin reacts with KLH. The RTK2071 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Trinitrophenol and KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    RTK2071
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use at an assay dependent dilution for cell surface or intracellular staining.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab18403 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Q&A

    Answer

    Thank you for your response.

    "No primary control" means that you perform the staining protocol as normal (fixation, blocking, secondary antibody, detection) - except you need to omit the incubation step with the primary antibody. Instead of applying the primary antibody, you only incubate the samples with the dilution buffer you would normally use for diluting the primary antibody.

    Isotype control meansyou perform the staining protocol as normal butt the samples will be treated with the isotype control instead of the primary antibody. The isotype of this primary antibody is IgG1, so the isotype control is rat IgG1 .

    I would advise you to visit this site to get some further information about positive control and isotype control:

    https://www.abcam.com/index.html?pageconfig=technicalfaqs

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Answer

    Thank you for your enquiry and your interest in our products.

    The immunogen used to raise this antibody was a synthetic peptide: SHGSLLLPPA SSPPPPQVPQ RRGTPPLTPG RLTQDLKL, corresponding to amino acids 1048-1085 of Human HCN4:SHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKL. Unfortunately, the peptide is not commercially available.

    Even though the immunogen peptide is not available, you could apply several controls to check the specificity of this antibody:

    - isotype control,

    - negative control,

    - no primary - only secondary control

    The isotype of this antibody is IgG1 so you can apply an isotype control using rat IgG1 (ab46745, ab128477, ab18403, ab18404, ab18407).

    HCN4 is highly expressed in thalamus, testis and in heart, both in ventricle and atrium. It is detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus. Generally, negative controls are cells/tissue which do not express the target protein or at very low level. You may find some useful information about expression level of HCN4 at this site: http://www.proteinatlas.org/ENSG00000138622

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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