Rat IgG1, kappa [RTK2071] - Isotype Control (Low endotoxin, Azide free) (ab18407)

Overview

  • Product name
    Rat IgG1, kappa [RTK2071] - Isotype Control (Low endotoxin, Azide free)
    See all Rat isotype controls
  • Specificity
    The RTK2071 immunoglobulin reacts with KLH. The RTK2071 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Functional Studies, Flow Cyt, IP, WBmore details
  • Immunogen

    Trinitrophenol and KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
    0.2 µm filter sterilized. Endotoxin level is <0.01 ng/µg protein.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Ab18407 is low endotoxin and azide free.
  • Clonality
    Monoclonal
  • Clone number
    RTK2071
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. For surface or intracellular staining.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

This product has been referenced in:
  • Raoufi-Rad N  et al. In vivo imaging of endothelial cell adhesion molecule expression after radiosurgery in an animal model of arteriovenous malformation. PLoS One 12:e0185393 (2017). Read more (PubMed: 28949989) »
  • He X  et al. The sst1 resistance locus regulates evasion of type I interferon signaling by Chlamydia pneumoniae as a disease tolerance mechanism. PLoS Pathog 9:e1003569 (2013). Read more (PubMed: 24009502) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Q&A

Answer

Thank you for your response.

"No primary control" means that you perform the staining protocol as normal (fixation, blocking, secondary antibody, detection) - except you need to omit the incubation step with the primary antibody. Instead of applying the primary antibody, you only incubate the samples with the dilution buffer you would normally use for diluting the primary antibody.

Isotype control meansyou perform the staining protocol as normal butt the samples will be treated with the isotype control instead of the primary antibody. The isotype of this primary antibody is IgG1, so the isotype control is rat IgG1 .

I would advise you to visit this site to get some further information about positive control and isotype control:

https://www.abcam.com/index.html?pageconfig=technicalfaqs

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for your enquiry and your interest in our products.

The immunogen used to raise this antibody was a synthetic peptide: SHGSLLLPPA SSPPPPQVPQ RRGTPPLTPG RLTQDLKL, corresponding to amino acids 1048-1085 of Human HCN4:SHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKL. Unfortunately, the peptide is not commercially available.

Even though the immunogen peptide is not available, you could apply several controls to check the specificity of this antibody:

- isotype control,

- negative control,

- no primary - only secondary control

The isotype of this antibody is IgG1 so you can apply an isotype control using rat IgG1 (ab46745, ab128477, ab18403, ab18404, ab18407).

HCN4 is highly expressed in thalamus, testis and in heart, both in ventricle and atrium. It is detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus. Generally, negative controls are cells/tissue which do not express the target protein or at very low level. You may find some useful information about expression level of HCN4 at this site: http://www.proteinatlas.org/ENSG00000138622

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question
Answer

Expiration date: 25-04-2012
I am very pleased to hear you would like to use ab18407 and ab18541 in ICC/IF and send us an image of your results.
This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview with the image for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.
If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your inquiry. I can confirm that the antibodies are all with kappa light chains as confirmed with ELISA assay. However, I do not have any information on whether they are 1a or 1b. Regarding the technical enquiry link, as I was able to access the enquiry page without difficulties, I believe that our IT department has spotted the problem and already resolved it. Should you face any inaccessibility again, we would be happy to hear about it. I am sorry I can't be of more assistance in this occasion. Please do not hesitate to contact us again should you require any further information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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