Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free) (ab18450)

Overview

  • Product name
    Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free)
    See all Rat isotype controls
  • Conjugation notes
    Please note that this antibody is unconjugated.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Frmore details
  • Immunogen

    Trinitrophenol + KLH

  • General notes

    ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
    0.2 µm filter sterilized. Endotoxin level is <0.01 ng/µg protein
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab18450 is LEAF™ Purified. This a low endotoxin – sodium azide free formulation. ab18450 was 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein. LEAF™ Purified formulation is typically used for bioassays, capture antibody for ELISPOT application, and in vivo studies such as Blocking/Neutralization.
  • Isotype control notes
    ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality
    Monoclonal
  • Clone number
    RTK2758
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells.

ab18450 is a negative isotype control.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

References

This product has been referenced in:
  • Shi Q  et al. The identification of the ATR inhibitor VE-822 as a therapeutic strategy for enhancing cisplatin chemosensitivity in esophageal squamous cell carcinoma. Cancer Lett 432:56-68 (2018). Read more (PubMed: 29890208) »
  • Chang TZ  et al. Effects of ovalbumin protein nanoparticle vaccine size and coating on dendritic cell processing. Biomater Sci 5:223-233 (2017). Read more (PubMed: 27918020) »
See all 6 Publications for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Question
Answer

Expiration date: 25-04-2012
I am very pleased to hear you would like to use ab18407 and ab18541 in ICC/IF and send us an image of your results.
This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview with the image for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.
If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your patience.

The subisotype ofab86488 is IgG2a. Thus, the recommended isotype control to be used in flow cytometry would be ab18450.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

Flow cytometry has not yet been tested with this antibody. Therefore, we do not have data to show if and how well it would work in this application. But for UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


As isotype control, I would suggest either

ab18450 (rat IgG2a) or ab18541 (rat IgG2b)
https://www.abcam.com/Rat-IgG2a-kappa-monoclonal-aRTK2758-ab18450.html
https://www.abcam.com/Rat-IgG2b-kappa-monoclonal-RTK4530-Isotype-control-ab18541.html

I will check with the lab if they have more information about the specific subisotype of this antibody (2a or 2b).



Please let me know if I shall issue a testing discount code for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today. - Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet). Please note that we will be unable to issue the credit note if the product is dispatched without the cooler box. However we are not able to accept products returned on dry ice. - Please mark on the documents the reason of return and Ref: - Please return using an over night courier. - You will need to provide the courier with an AirWay Bill – which you can get from the courier, and fill out with our details. - Our address is: Abcam plc, 330 Cambridge Science Park, Milton Road, Cambridge, CB4 0FL When we have received the goods, we will process the return. We will then raise a credit note for the invoice less 20% restocking fee. If you have already paid this invoice, we will reallocate this money to a new invoice for a future order and if there’s any difference in price, we will refund or re-invoice accordingly. For the correct item, please place a new order in the normal methods. Please confirm if you are planning to return the product to us. Important: if the product is not returned to Abcam within next 7 days we will cancel the credit note. PS: We have anti IgG2a and anti IgG2c available in catalogue that are currently in stock. These 2 antibodies exactly match your request. The catalogue numbers are: ab18446 and ab114134

Read More

Question
Answer

Thank you for your enquiry. To our knowledge this antibody has yet to be tested in-vivo. If you decide to purchase this antibody for testing in applications for which we don't have any information, please let us know how you get on. In return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up