violetFluor™ 450 Rat IgG2a, monoclonal [2A3] - Isotype Control (ab253065)
Overview
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Product name
violetFluor™ 450 Rat IgG2a, monoclonal [2A3] - Isotype Control
See all IgG2a isotype controls -
Conjugation
violetFluor™ 450. Ex: 405nm, Em: 450nm -
Specificity
ab253065 binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells.
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Tested applications
Suitable for: Flow Cytmore details -
Immunogen
Chemical/ Small Molecule. TNP hapten.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
2A3 -
Isotype
IgG2a -
Research areas
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Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens. -
Cellular localization
Secreted
Associated products
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Alternative Versions
- APC Rat IgG2a, monoclonal [2A3] - Isotype control (ab253040)
- APC/Cy7® Rat IgG2a, monoclonal [2A3]- Isotype control (ab253043)
- PE Rat IgG2a, monoclonal [2A3] - Isotype Control (ab253047)
- PE/Cy5® Rat IgG2a, monoclonal [2A3] (PE/Cy5® ) - Isotype Control (ab253049)
- PE/Cy7® Rat IgG2a, monoclonal [2A3] - Isotype Control (ab253051)
- PerCP/Cy5.5® Rat IgG2a, monoclonal [2A3] - Isotype Control (ab253054)
- redFluor™ 710 Rat IgG2a, monoclonal [2A3] - Isotype control (ab253074)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
This antibody preparation has been quality-tested for flow cytometry using an appropriate cell type. The isotype control should be used at the same concentration as the primary antibody for which it is a control. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. This antibody preparation has been quality-tested for flow cytometry using an appropriate cell type. The isotype control should be used at the same concentration as the primary antibody for which it is a control. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab253065 has not yet been referenced specifically in any publications.