Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)

Overview

  • Product name
    Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control
    See all Rat isotype controls
  • Tested applications
    Suitable for: ICC, IHC-P, WB, IP, Flow Cytmore details
  • Immunogen

    Trinitrophenol and KLH

  • General notes
    Isotype control has been screened on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    RTK4530
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution. This product is also suitable for use in intracellular flow cytometry.

References

This product has been referenced in:
  • Raoufi-Rad N  et al. In vivo imaging of endothelial cell adhesion molecule expression after radiosurgery in an animal model of arteriovenous malformation. PLoS One 12:e0185393 (2017). Read more (PubMed: 28949989) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Answer

Thank you for contacting us again.
For price and shipping please contact our distributor Sapphire via our website.
There are two types of light chain in humans (as in other mammals),
kappa (κ) chain, encoded by the immunoglobulin kappa locus (IGK@) on chromosome 2 and lambda (λ) chain, encoded by the immunoglobulin lambda locus (IGL@) on chromosome 22

Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte. In a healthy individual, the total kappa to lambda ratio is roughly 2:1 in serum (measuring intact whole antibodies) or 1:1.5 if measuring free light chains, with a highly divergent ratio indicative of neoplasm.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Unfortunately ab18536 and ab18541 have not been tested in ChIP. It doesn’t mean they won’t work in this application, but we don’t have data to support it.

If you are interested in participating in our antibodies evaluation system (Abreview), and test any of these antibodies with ChIP I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

Therefore, I can offer a discount off a future purchase if you buy an antibody and test it in the new application and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test either (or both) of the antibodies in ChIP. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

3. Test it in the new application.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

Please notice these are isotype control antibodies, they will not bind Rat IgG2b, but they will confirm the primary antibody is binding to the specific target, and not randomly performing non specific Fc receptor binding.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Flow cytometry has not yet been tested with this antibody. Therefore, we do not have data to show if and how well it would work in this application. But for UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


As isotype control, I would suggest either

ab18450 (rat IgG2a) or ab18541 (rat IgG2b)
https://www.abcam.com/Rat-IgG2a-kappa-monoclonal-aRTK2758-ab18450.html
https://www.abcam.com/Rat-IgG2b-kappa-monoclonal-RTK4530-Isotype-control-ab18541.html

I will check with the lab if they have more information about the specific subisotype of this antibody (2a or 2b).



Please let me know if I shall issue a testing discount code for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Ab18541 is an isotype control i.e a Rat IgG2b, kappa monoclonal antibody, not an antibody against a specific protein which would be expressed in some tissues. Can I please check the catalogue number you are enquiring as this product does not have positive controls?

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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