For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
|Sample type||Average %||Range|
|Serum||> 85||% - %|
Abcam’s IgM Rat ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgM in rat serum and plasma samples.
In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|100X HRP-conjugated anti-rat IgM antibody||1 x 150µl|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Chromogen Substrate Solution||1 x 12ml|
|Rat IgM Calibrator||1 vial|
|Rat IgM ELISA Microplate||1 unit|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab157738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"