Overview

  • Product name

    Rat IL-1 alpha ELISA Kit
    See all IL-1 alpha kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 3 2.7%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 8 2.9%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.205 pg/ml
  • Range

    5.47 pg/ml - 350 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 112 101% - 121%
    Serum 98 96% - 103%
    Heparin Plasma 97 97% - 98%
    EDTA Plasma 92 90% - 93%
    Citrate Plasma 99 96% - 104%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    IL-1 alpha in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 alpha protein in rat serum, plasma, and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Interleukin 1 (IL-1) refers to two proteins encoded by two different genes (IL-1a and IL-1b), both of which bind the cell surface receptor IL-1R. IL-1a is a proinflammatory cytokine typically expressed under normal conditions in skin keratinocytes, epithelial cells as well as cells of the central nervous system, monocytes, tissue macrophages, dendritic cells, B lymphocytes and NK cells. IL-1a is synthesized as a 31kDa precursor and cleaved into a 17.5kDa protein. IL-1 receptor signaling induces recruitment of IL-1 R accessory protein leading to phosphorylation and ubiquitination events that ultimately activate NF-?B and signal via JNK and MAPK pathways. These signaling events generate local and systemic responses mediating acute phase inflammation inducing fever, pain sensitivity, vasodilation. However, prolonged and inappropriate IL-1 induction has been shown to be associated with sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus and atherosclerosis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat IL-1 alpha Capture Antibody 1 x 600µl
    10X Rat IL-1 alpha Detector Antibody 1 x 600µl
    Rat IL-1 alpha Lyophilized Recombinant Protein 2 vials
    Antibody Diluent 4BR 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similarities

    Belongs to the IL-1 family.
  • Domain

    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localization

    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Alternative names

    • BAF
    • FAF
    • Hematopoietin 1
    • Hematopoietin-1
    • IL 1 alpha
    • IL 1A
    • IL-1 alpha
    • Il-1a
    • IL1
    • IL1 ALPHA
    • IL1A
    • IL1A_HUMAN
    • IL1F1
    • Interleukin 1 alpha
    • Interleukin-1 alpha
    • Interleukin1 alpha
    • LAF
    • LEM
    • Preinterleukin 1 alpha
    • Pro interleukin 1 alpha
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab235646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between Rat IL-1 alpha SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • The concentrations of IL-1 alpha were measured in duplicates, interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted sample was rat spleen cell supernatant 100%. Cells were treated with 100 ng/mL LPS in RPMI culture media containing glutaMAX and 10% fetal bovine serum over 3 days. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 alpha concentration was determined to be 251.71 pg/mL in LPS treated spleen cell supernatant 100%.

  • Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab235646 has not yet been referenced specifically in any publications.

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