Overview

  • Product name

    Rat IL-10 ELISA Kit
    See all IL-10 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.69 90% - 109%
    Serum 96.75 84% - 104%
    Plasma 95.97 82% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s IL-10 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-10 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100764.

  • Representative standard curve using ab100764.

Protocols

References

This product has been referenced in:

  • Piao JM  et al. MicroRNA-381 Favors Repair of Nerve Injury Through Regulation of the SDF-1/CXCR4 Signaling Pathway via LRRC4 in Acute Cerebral Ischemia after Cerebral Lymphatic Blockage. Cell Physiol Biochem 46:890-906 (2018). Read more (PubMed: 29669322) »
  • Yang Y  et al. The Anti-Inflammatory Effect of Guchangzhixie-Pill by Reducing Colonic EC Cell Hyperplasia and Serotonin Availability in an Ulcerative Colitis Rat Model. Evid Based Complement Alternat Med 2017:8547257 (2017). Read more (PubMed: 28845184) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.

I now have further information to share with you in regards to which IL10 and TNF alpha kits we have which may be suitable for the application you wish to use them with.

Having looked through our catalogue I would recommend trying the IL10 kit (ab100764) and TNF alpha kit (ab100784). Although we have not tried them ourselves with bronchoalveolar lavage samples, but these kits should be applicable, although as we have not tried it ourselves I would not be able to guarantee it. The fluid collected shouldn't need to be prepared any differently to serum or culture supernatant as described in the protocol booklets of these kits.

I have found a few references which may be of use to you in regards to the experiment:

http://ajrccm.atsjournals.org/content/176/2/146.full

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2008.03832.x/full

It seems that generally these kind of samples are used undiluted, but if you find the signal too strong you can dilute them using the Assay diluent provided to dilute cell culture media samples.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up