Overview

  • Product name

    Rat IL-10 ELISA Kit
    See all IL-10 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    140 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 142.1 133% - 149%
    Tissue Extracts 138.7 127% - 146%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s IL-10 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-10 in cell lysates and tissue lysates.

    This assay employs an antibody specific for rat IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100765.

Protocols

References

This product has been referenced in:

  • Hassan I  et al. Copper Mediates Anti-Inflammatory and Antifibrotic Activity of Gleevec in Hepatocellular Carcinoma-Induced Male Rats. Can J Gastroenterol Hepatol 2019:9897315 (2019). Read more (PubMed: 30941331) »
  • Fahmi AA  et al. Chemical composition and protective role of Pulicaria undulata (L.) C.A. Mey. subsp. undulata against gastric ulcer induced by ethanol in rats. Heliyon 5:e01359 (2019). Read more (PubMed: 30957042) »
See all 13 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.
I am sorry that the standard curve was non-linear in the kit you received. Can you please confirm some additional details?
1) Was the vial of Item C spun prior to opening? Did you mix thoroughly to resuspend the standard in the sample diluent buffer?
2) What OD readings were you getting for your blank and standards? It would be very helpful if you could please send the raw data from your plate.
3) Can you please provide the original order or PO number?
I will be happy to help you further once I have this additional information. If the kit is not working as stated on our datasheet and you have purchased it in the past six months I will be happy to offer you a free of charge replacement or refund.

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