Question (38553) | Rat IL-10 ELISA Kit (ab100765)

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Question

LOT NUMBER GR37065-1 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Problem with standard curve SAMPLE rat brain PRIMARY ANTIBODY immobilized antibody DETECTION METHOD TMB in buffered solution ANTIBODY STORAGE CONDITIONS + 4 °C TYPE OF ELISA Sandwich ELISA BLOCKING CONDITIONS 0.2 M sulfuric acid SECONDARY ANTIBODY biotinylated anti rat IL10 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES OD of the standard curve dilutions were similar to the Blank OD. It seems like no IL10 was present in any of the two vials provided.

Answer

Thank you for contacting us.
I am sorry that the standard curve was non-linear in the kit you received. Can you please confirm some additional details?
1) Was the vial of Item C spun prior to opening? Did you mix thoroughly to resuspend the standard in the sample diluent buffer?
2) What OD readings were you getting for your blank and standards? It would be very helpful if you could please send the raw data from your plate.
3) Can you please provide the original order or PO number?
I will be happy to help you further once I have this additional information. If the kit is not working as stated on our datasheet and you have purchased it in the past six months I will be happy to offer you a free of charge replacement or refund.

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