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RecombinantRabMAb

Rat IL-13 Antibody Pair - BSA and Azide free (ab270384)

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Sandwich ELISA - Rat IL-13 Antibody Pair - BSA and Azide free (ab270384)

    Key features and details

    • Unconjugated capture and detector antibodies
    • Adaptable to any antibody pair-based assay format
    • Antibody concentration ~ 1 mg/ml
    • BSA and azide free buffer - ready for conjugation
    • Reacts with: Rat

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    Overview

    • Product name

      Rat IL-13 Antibody Pair - BSA and Azide free
      See all IL-13 kits
    • Assay type

      ELISA set
    • Range

      78.125 ng/ml - 5000 ng/ml
    • Species reactivity

      Reacts with: Rat
    • Product overview

      The Antibody Pair can be used to quantify Rat IL-13. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


      For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab269547), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


      Download SDS here.

    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Carrier free

      Yes
    • Components 10 x 96 tests
      Rat IL-13 Capture Antibody (unconjugated) 1 x 100µg
      Rat IL-13 Detector Antibody (unconjugated) 1 x 100µg
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers
    • Function

      Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
    • Involvement in disease

      Allergic rhinitis
    • Sequence similarities

      Belongs to the IL-4/IL-13 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P35225 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Allergic rhinitis
      • ALRH
      • BHR 1
      • BHR1
      • Bronchial hyperresponsiveness 1 (bronchial asthma)
      • IL 13
      • IL-13
      • Il13
      • IL13_HUMAN
      • Interleukin 13
      • Interleukin-13
      • interleukin13
      • MGC116786
      • MGC116788
      • MGC116789
      • NC 30
      • NC30
      • P 600
      • P600
      see all
    • Database links

      • Entrez Gene: 116553 Rat
      • SwissProt: P42203 Rat
      • Unigene: 9921 Rat

      Associated products

      • Alternative Versions

        • Rat IL-13 ELISA Kit (ab269547)

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab270384 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Sandwich ELISA
      Use at an assay dependent concentration.
      Notes
      Sandwich ELISA
      Use at an assay dependent concentration.

      Images

      • Sandwich ELISA - Rat IL-13 Antibody Pair - BSA and Azide free (ab270384)
        Sandwich ELISA - Rat IL-13 Antibody Pair - BSA and Azide free (ab270384)
        To learn more about the advantages of recombinant antibodies see here.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab270384? Please let us know so that we can cite the reference in this datasheet.

      ab270384 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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