Rat IL-13 ELISA Kit (ab100766)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-13 ELISA Kit
See all IL-13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
27.43 pg/ml - 20000 pg/ml -
Recovery
114 %
Sample specific recovery Sample type Average % Range Cell culture extracts 120.4 80% - 139% Tissue Extracts 107.8 81% - 135% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s IL-13 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-13 cell lysate and tissue lysate.
This assay employs an antibody specific for Rat IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-rat IL-13 2 vials IL-13 Microplate (12 x 8 wells) 1 unit Recombinant Rat IL-13 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Allergic rhinitis
- ALRH
- BHR 1
see all -
Database links
- Entrez Gene: 116553 Rat
- SwissProt: P42203 Rat
- Unigene: 9921 Rat
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab100766 has been referenced in 5 publications.
- Jing L et al. Srolo Bzhtang, a traditional Tibetan medicine formula, inhibits cigarette smoke induced airway inflammation and muc5ac hypersecretion via suppressing IL-13/STAT6 signaling pathway in rats. J Ethnopharmacol 235:424-434 (2019). PubMed: 30731182
- Yan M et al. The Mechanism of Electroacupuncture at Zusanli Promotes Macrophage Polarization during the Fibrotic Process in Contused Skeletal Muscle. Eur Surg Res 60:196-207 (2019). PubMed: 31694021
- Iturra PA et al. Progression of Type 2 Helper T Cell-Type Inflammation and Airway Remodeling in a Rodent Model of Naturally Acquired Subclinical Primary Pneumocystis Infection. Am J Pathol 188:417-431 (2018). PubMed: 29169991
- Seiffert J et al. Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726 (2015). Rat . PubMed: 25747867
- Qin WY et al. Electroacupuncture Could Regulate the NF-?B Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats. Evid Based Complement Alternat Med 2013:924541 (2013). PubMed: 23970940