SimpleStep

Rat IL-13 ELISA Kit (ab269547)

Submit a review Submit a question

Overview

  • Product name

    Rat IL-13 ELISA Kit
    See all IL-13 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2%

  • Sample type

    Urine, Serum, Cell culture media, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    20.03 pg/ml

  • Range

    78.13 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 83 80% - 86%
    Serum 87 81% - 92%
    Cell culture media 90 84% - 98%
    EDTA Plasma 97 84% - 117%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Rat IL-13 ELISA Kit (ab269547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-13 protein in edta plasma, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-13 with 20.03 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL-2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure. The antibodies for this kit were raised against a recombinant peptide fragment of the mature rat protein.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of rat IL-13 standard curve in Sample Diluent 50BP.
    Example of rat IL-13 standard curve in Sample Diluent 50BP.

    The IL-13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.
    Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.

    The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: rat serum 50%, rat plasma (EDTA) 50%, rat urine 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

References

Publishing research using ab269547? Please let us know so that we can cite the reference in this datasheet.

ab269547 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab269547.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com