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    rat-il-13-elisa-kit-ab269547.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantSimpleStep

Rat IL-13 ELISA Kit (ab269547)

  • Datasheet
  • SDS
  • Protocol Booklet
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SimpleStep ELISA Protocol Diagram
  • Example of rat IL-13 standard curve in Sample Diluent 50BP.
  • Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.
  • Sandwich ELISA - Rat IL-13 ELISA Kit (ab269547)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 20.03 pg/ml
  • Range: 78.13 pg/ml - 5000 pg/ml
  • Sample type: Cell culture media, EDTA Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

You may also be interested in

Protein
Recombinant rat IL-13 protein (ab51960)

View more associated products

Overview

  • Product name

    Rat IL-13 ELISA Kit
    See all IL-13 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2%
  • Sample type

    Urine, Serum, Cell culture media, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    20.03 pg/ml
  • Range

    78.13 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 83 80% - 86%
    Serum 87 81% - 92%
    Cell culture media 90 84% - 98%
    EDTA Plasma 97 84% - 117%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Rat IL-13 ELISA Kit (ab269547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-13 protein in edta plasma, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-13 with 20.03 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL-2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure. The antibodies for this kit were raised against a recombinant peptide fragment of the mature rat protein.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat IL-13 Capture Antibody 1 x 600µl
    10X Rat IL-13 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Plate Seals 1 unit
    Rat IL-13 Lyophilized Recombinant Protein 2 vials
    Sample Diluent 50BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
  • Function

    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease

    Allergic rhinitis
  • Sequence similarities

    Belongs to the IL-4/IL-13 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P35225 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Allergic rhinitis
    • ALRH
    • BHR 1
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • interleukin13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC 30
    • NC30
    • P 600
    • P600
    see all
  • Database links

    • Entrez Gene: 116553 Rat
    • SwissProt: P42203 Rat
    • Unigene: 9921 Rat

    Associated products

    • Alternative Versions

      • Rat IL-13 Antibody Pair - BSA and Azide free (ab270384)

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of rat IL-13 standard curve in Sample Diluent 50BP.
      Example of rat IL-13 standard curve in Sample Diluent 50BP.

      The IL-13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.
      Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.

      The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: rat serum 50%, rat plasma (EDTA) 50%, rat urine 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Sandwich ELISA - Rat IL-13 ELISA Kit (ab269547)
      Sandwich ELISA - Rat IL-13 ELISA Kit (ab269547)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab269547? Please let us know so that we can cite the reference in this datasheet.

    ab269547 has not yet been referenced specifically in any publications.

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