• Product name
    Rat IL-17A ELISA Kit (Interleukin-17)
    See all IL17A kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 7 8.5%
    Sample n Mean SD CV%
    Overall 7 7.6%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1 pg/ml
  • Range
    1.6 pg/ml - 100 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92% - 112%
    Serum 34% - 44%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-17A (Interleukin-17) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed

    for accurate quantitative measurement of Rat IL-17 (Interleukin-17)concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    IL-17 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-17 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17 captured on the plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-rat IL17 monoclonal antibody 1 x 70µl
    Microplate coated with monoclonal antibody to rat IL-17 (12 x 8 wells) 1 unit
    Rat IL17 Standard Lyophilized (200 pg/mL on reconstitution) 2 vials
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Function
    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity
    Restricted to activated memory T-cells.
  • Sequence similarities
    Belongs to the IL-17 family.
  • Post-translational
    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • OTTHUMP00000016597
    see all
  • Database links


Our Abpromise guarantee covers the use of ab119536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab119536.



This product has been referenced in:
  • Liu Z  et al. Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats. Evid Based Complement Alternat Med 2018:3674145 (2018). Read more (PubMed: 29541141) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your patience in this matter. There will be a big difference in the height of the OD values measured before and after addition of the stop solution, so you should take your readings AFTER the stop solution.

I would also recommen...

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Samples can vary from mouse to mouse because all mice (even within the same stains) to do not always express proteins at the same levels. Therefore a titration may be necessary.

However, if instructions in our protocol have been followed,...

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Thank you for your reply.

The Anti-PPAR gamma antibody (ab27649) is provided already reconstituted in liquid form in a PBS buffer (pH 7.4) with 1% BSA and 0.02% sodium azide supplemented. The customer therefore does not need to reconstitute i...

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Muchas gracias por contactarnos. Agradezco el interés en nuestros productos.

Lamentablemente, ya que tenemos en el catálogo más de 80.000 productos, nos resulta imposible guardar muestras de prueba de todos ellos para enviar como alícuotas d...

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