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Thank you for the certificates. However i need more info on this kit and ab.
Please advise on expiry dates of these and advise on the use of ab27649. It should be resuspended in sterile PBS, but please specify which amount and in such manner that 100 tests can be done with it.
Asked on Aug 29 2012
Thank you for your reply.
The Anti-PPAR gamma antibody (ab27649) is provided already reconstituted in liquid form in a PBS buffer (pH 7.4) with 1% BSA and 0.02% sodium azide supplemented. The customer therefore does not need to reconstitute it. I am sorry but I do not think I fully understand the question in regards to how many tested can be performed with this antibody. We would advise trying the antibody at a dilution of 1/1000 for Western blotting and 1/200 for IHC. However these are only guidelines and further optimisation of the protocol may be required to obtain the best results.
We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody and kit. These conditions will vary among our products, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 120 days from the date of purchase when stored correctly.
For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).
I hope this information helps. Please do not hesitate to contact us if you need anything further.
Answered on Aug 29 2012