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what suggested dilution to use with plasma or serum samples?
Asked on Apr 15 2013
Samples can vary from mouse to mouse because all mice (even within the same stains) to do not always express proteins at the same levels. Therefore a titration may be necessary.
However, if instructions in our protocol have been followed, samples have been diluted 1:2 (50ul sample + 50ul Sample Diluent), the concentration read from the standard curve must be multiplied by the dilution factor (X2).
Answered on Apr 15 2013