Overview

  • Product name

    Rat IL-17A ELISA Kit
    See all IL-17A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5 7%
  • Sample type

    Cell culture supernatant, Serum, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.1 pg/ml
  • Range

    6.25 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 113 105% - 117%
    Cell culture media 101 96% - 110%
    Citrate Plasma 110 108% - 112%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Human
  • Product overview

    IL-17A in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A protein in rat serum, plasma, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS: 1.1 pg/mL
    Samples in Sample Diluent 75BS: 3.2 pg/mL

  • Notes

    IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat IL-17a Capture Antibody 1 x 600µl
    10X Rat IL-17a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BR 1 x 6ml
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Rat IL-17a Lyophilized Recombinant Protein 2 vials
    Sample Diluent 75BS 1 x 20ml
    Sample Diluent NS 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • interleukin17
    • Interleukin17A
    • OTTHUMP00000016597
    • OTTMUSP00000046003
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab214028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Rat spleen cells were cultured in the absence or presence of 5 µg/mL Concanavalin A for 48 hours. The concentrations of Il-17a were measured in neat supernatant samples in duplicates and interpolated from the IL-17a standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean IL‑17a concentration was determined to be 33.2 pg/mL in unstimulated, 187 pg/mL in Concanavalin A stimulated supernatants and undetectable in media (not shown).

  • The concentrations of IL-17a were measured in duplicates, interpolated from the IL-17a standard curves and corrected for sample dilution. Undiluted samples are as follows: rat spleen cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17a concentration was determined to be 190 pg/mL in Concanavalin A treated rat spleen cell culture supernatant.

  • The concentrations of IL-17a were measured in duplicates, interpolated from the IL-17a standard curves and corrected for sample dilution. Undiluted samples are as follows: rat serum and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Background-subtracted data values (n = 1) are graphed. Note that this kit is specific for IL-17a homodimer, it does not recognize IL-17f homodimer or IL-17a/f heterodimer.

  • Two fold serial dilutions of rat, mouse and human recombinant IL-17a proteins were measured with this kit. Background-subtracted data values (mean +/- SD, n = 2) are graphed. O.D. values for human IL-17a protein were below background values. Note this kit reacts with mouse recombinant IL-17a protein. Note this kit does not react with human recombinant IL-17a protein.

  • Background-subtracted data values (mean +/- SD) are graphed

Protocols

References

This product has been referenced in:

  • Hu X  et al. RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll-like receptor signaling pathway. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 31012109) »
  • Feng F  et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). ELISA ; Rat . Read more (PubMed: 30546393) »
See all 2 Publications for this product

Customer reviews and Q&As

simplestep

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit is very easy to use, the whole process takes less than 2 hours to produce results, and the operation is very simple, much easier than I have used a conventional ELISA kit. This kit is highly recommended.

Abcam user community

Verified customer

Submitted Aug 09 2019

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