Recombinant
SimpleStep

Rat IL-17A ELISA Kit (ab214028)

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Overview

  • Product name

    Rat IL-17A ELISA Kit
    See all IL-17A kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5 7%

  • Sample type

    Cell culture supernatant, Serum, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.1 pg/ml

  • Range

    6.25 pg/ml - 400 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 113 105% - 117%
    Cell culture media 101 96% - 110%
    Cit plasma 110 108% - 112%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Rat IL-17A ELISA Kit (ab214028) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17A protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-17A with 1.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat IL-17a Capture Antibody 1 x 600µl
    10X Rat IL-17a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BR 1 x 6ml
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Rat IL-17a Lyophilized Recombinant Protein 2 vials
    Sample Diluent 75BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.

  • Tissue specificity

    Restricted to activated memory T-cells.

  • Sequence similarities

    Belongs to the IL-17 family.

  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • interleukin17
    • Interleukin17A
    • OTTHUMP00000016597
    • OTTMUSP00000046003
    see all

  • Database links

    Associated products

    Images

    • Other - Rat IL-17A ELISA Kit (ab214028)
      Other - Rat IL-17A ELISA Kit (ab214028)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of rat IL-17a standard curve in Sample Diluent NS.
      Example of rat IL-17a standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Comparison of IL-17a in unstimulated and Concanavalin A stimulated rat spleen cell supernatants.
      Comparison of IL-17a in unstimulated and Concanavalin A stimulated rat spleen cell supernatants.

      Rat spleen cells were cultured in the absence or presence of 5 µg/mL Concanavalin A for 48 hours. The concentrations of Il-17a were measured in neat supernatant samples in duplicates and interpolated from the IL-17a standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean IL‑17a concentration was determined to be 33.2 pg/mL in unstimulated, 187 pg/mL in Concanavalin A stimulated supernatants and undetectable in media (not shown).

    • Interpolated concentrations of native IL-17a in 2 days 5 µg/mL Concanavalin A treated rat spleen cell culture supernatant samples.
      Interpolated concentrations of native IL-17a in 2 days 5 µg/mL Concanavalin A treated rat spleen cell culture supernatant samples.

      The concentrations of IL-17a were measured in duplicates, interpolated from the IL-17a standard curves and corrected for sample dilution. Undiluted samples are as follows: rat spleen cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17a concentration was determined to be 190 pg/mL in Concanavalin A treated rat spleen cell culture supernatant.

    • Serial dilutions of recombinant rat IL-17a homodimer, recombinant mouse IL-17f homodimer and recombinant mouse IL-17a/f heterodimer were measured with this kit.
      Serial dilutions of recombinant rat IL-17a homodimer, recombinant mouse IL-17f homodimer and recombinant mouse IL-17a/f heterodimer were measured with this kit.

      Background-subtracted data values (n = 1) are graphed. Note that this kit is specific for IL-17a homodimer, it does not recognize IL-17f homodimer or IL-17a/f heterodimer.

    • Titration of mouse, rat and human recombinant IL-17a within the working range of the assay.
      Titration of mouse, rat and human recombinant IL-17a within the working range of the assay.

      Two fold serial dilutions of rat, mouse and human recombinant IL-17a proteins were measured with this kit. Background-subtracted data values (mean +/- SD, n = 2) are graphed. O.D. values for human IL-17a protein were below background values. Note this kit reacts with mouse recombinant IL-17a protein. Note this kit does not react with human recombinant IL-17a protein.

    • Example of rat IL-17a standard curve in Sample Diluent 75BS.
      Example of rat IL-17a standard curve in Sample Diluent 75BS.

      Background-subtracted data values (mean +/- SD) are graphed

    References (2)

    Publishing research using ab214028? Please let us know so that we can cite the reference in this datasheet.

    ab214028 has been referenced in 2 publications.

    • Hu X  et al. RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll-like receptor signaling pathway. J Cell Physiol N/A:N/A (2019). PubMed: 31012109
    • Feng F  et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). ELISA ; Rat . PubMed: 30546393

    Customer reviews and Q&As

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    simplestep

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    This kit is very easy to use, the whole process takes less than 2 hours to produce results, and the operation is very simple, much easier than I have used a conventional ELISA kit. This kit is highly recommended.

    Abcam user community

    Verified customer

    Submitted Aug 09 2019

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