Overview

  • Product name

    Rat IL-17F ELISA Kit
    See all IL-17F kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    splenocyte 8 2.3%
    Inter-assay
    Sample n Mean SD CV%
    splenocyte 3 2.1%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2 pg/ml
  • Range

    9.4 pg/ml - 800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 91% - 115%
    Serum 89 87% - 92%
    Cell culture media 102 97% - 108%
    Hep Plasma 90 87% - 95%
    EDTA Plasma 122 112% - 130%
    Cit plasma 107 106% - 108%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    IL-17F in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17F protein in ratserum, plasma, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IL-17F is an IL-17 family member cytokine that stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. IL-17F inhibits angiogenesis. IL-17F is a glycosylated, secreted disulfide-linked homodimer. IL-17F is also present as IL-17A/F heterodimer. IL-17F is expressed in activated, but not resting, CD4+ T-cells and activated monocytes.


    Sensitivity:


    Samples diluted in Sample Diluent NS: 2.0 pg/mL


    Samples diluted Sample Diluent 50BP: 2.7 pg/mL

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat IL-17F Capture Antibody 1 x 600µl
    10X Rat IL-17F Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BR 1 x 6ml
    Plate Seals 1 unit
    Rat IL-17F Lyophilized Recombinant Protein 2 vials
    Sample Diluent 50BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
  • Tissue specificity

    Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
  • Involvement in disease

    Candidiasis, familial, 6
  • Sequence similarities

    Belongs to the IL-17 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CANDF6
    • Cytokine ML 1
    • Cytokine ML-1
    • IL 17F
    • IL 24
    • IL-17F
    • IL-24
    • Il17f
    • IL17F_HUMAN
    • Interleukin 17F
    • Interleukin 24
    • Interleukin-17F
    • Interleukin-24
    • ML 1
    • ML1
    • Mutant IL 17F
    • OTTHUMP00000016602
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab239423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between Rat IL-17F SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
  • The IL-17F standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • The IL-17F standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • Rat splenocyte cells were cultured in the presence (treated) or absence (untreated) of 10 ng/mL TPA and 10 ng/mL rat IL-1a for four days. The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curves and corrected for sample dilution. Undiluted samples are as follows: treated splenocyte supernatant 5%, and untreated splenocyte supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17F concentration was determined to be 6,477 pg/mL in neat treated splenocyte supernatant, and 326.4 ng/mL in neat untreated splenocyte supernatant.
  • Background subtracted data values (mean +/- SD, n=2) are graphed. Note that the IL-17A protein is not detectable with this kit in the range assayed.

Protocols

References

ab239423 has not yet been referenced specifically in any publications.

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