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Immunology Innate Immunity Cytokines Interleukins
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Rat IL-18 ELISA Kit (ab213909)

  • Datasheet
  • SDS
  • Protocol Booklet
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Rat IL-18 ELISA Kit (ab213909) Standard Curve

    Key features and details

    • Sensitivity: 1 pg/ml
    • Range: 15.6 pg/ml - 1000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

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    Overview

    • Product name

      Rat IL-18 ELISA Kit
      See all IL-18 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Sample 1 16 29pg/ml 1.97 6.8%
      Sample 2 16 166pg/ml 2.2 4.7%
      Sample 3 16 511pg/ml 22.99 4.5%
      Inter-assay
      Sample n Mean SD CV%
      Sample 1 24 29pg/ml 2.2 7.6%
      Sample 2 24 165pg/ml 9.07 5.5%
      Sample 3 24 490pg/ml 29.4 6%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 1 pg/ml
    • Range

      15.6 pg/ml - 1000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Rat
    • Product overview

      The Rat IL-18 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213909) is designed for the quantitative measurement of Rat IL-18 in cell culture supernatants, serum and plasma (EDTA). 


      The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IL-18 amount of sample captured in plate.

    • Notes

      Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.  IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-rat IL-18 coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti- Rat IL-18 antibody 1 x 130µl
      Lyophilized recombinant Rat IL-18 standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
    • Function

      Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
    • Sequence similarities

      Belongs to the IL-1 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q14116 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Iboctadekin
      • IFN gamma inducing factor
      • IFN-gamma-inducing factor
      • IGIF
      • IL 1 gamma
      • IL 18
      • IL 1g
      • IL-1 gamma
      • IL-18
      • IL1 gamma
      • IL18
      • IL18 protein
      • IL18_HUMAN
      • IL1F4
      • IL1g
      • IL1gamma
      • ILIF4
      • Interferon gamma inducing factor
      • Interferon gamma-inducing factor
      • Interleukin 1 gamma
      • Interleukin 18
      • Interleukin 18 (interferon-gamma-inducing factor)
      • Interleukin-1 gamma
      • Interleukin-18
      • Interleukin18
      • MGC12320
      see all
    • Database links

      • Entrez Gene: 29197 Rat
      • SwissProt: P97636 Rat
      • Unigene: 11118 Rat

      Associated products

        Images

        • Rat IL-18 ELISA Kit (ab213909) Standard Curve
          Rat IL-18 ELISA Kit (ab213909) Standard Curve

          Rat IL-18 ELISA Kit (ab213909) Standard Curve

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (2)

        Publishing research using ab213909? Please let us know so that we can cite the reference in this datasheet.

        ab213909 has been referenced in 2 publications.

        • Cornelius DC  et al. NLRP3 inflammasome activation in platelets in response to sepsis. Physiol Rep 7:e14073 (2019). PubMed: 31054188
        • Lin ZH  et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia. Front Cell Neurosci 11:391 (2017). PubMed: 29311826

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