Rat IL-18 ELISA Kit (ab213909)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-18 ELISA Kit
See all IL-18 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample 1 16 29pg/ml 1.97 6.8% Sample 2 16 166pg/ml 2.2 4.7% Sample 3 16 511pg/ml 22.99 4.5% Inter-assay Sample n Mean SD CV% Sample 1 24 29pg/ml 2.2 7.6% Sample 2 24 165pg/ml 9.07 5.5% Sample 3 24 490pg/ml 29.4 6% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
The Rat IL-18 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213909) is designed for the quantitative measurement of Rat IL-18 in cell culture supernatants, serum and plasma (EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IL-18 amount of sample captured in plate.
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Notes
Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses. IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat IL-18 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Rat IL-18 antibody 1 x 100µl Lyophilized recombinant Rat IL-18 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Iboctadekin
- IFN gamma inducing factor
- IFN-gamma-inducing factor
see all -
Database links
- Entrez Gene: 29197 Rat
- SwissProt: P97636 Rat
- Unigene: 11118 Rat
Images
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Rat IL-18 ELISA Kit (ab213909) Standard Curve
Rat IL-18 ELISA Kit (ab213909) Standard Curve
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab213909 has been referenced in 10 publications.
- Zhao H et al. Propofol ameliorates endotoxin-induced myocardial cell injury by inhibiting inflammation and apoptosis via the PPAR?/HMGB1/NLRP3 axis. Mol Med Rep 23:N/A (2021). PubMed: 33398367
- Yang L et al. Azeliragon ameliorates Alzheimer's disease via the Janus tyrosine kinase and signal transducer and activator of transcription signaling pathway. Clinics (Sao Paulo) 76:e2348 (2021). PubMed: 33681944
- Mohamed RA et al. Palonosetron/Methyllycaconitine Deactivate Hippocampal Microglia 1, Inflammasome Assembly and Pyroptosis to Enhance Cognition in a Novel Model of Neuroinflammation. Molecules 26:N/A (2021). PubMed: 34443654
- Liang Y et al. Inhibition of Caspase-1 Ameliorates Ischemia-Associated Blood-Brain Barrier Dysfunction and Integrity by Suppressing Pyroptosis Activation. Front Cell Neurosci 14:540669 (2020). PubMed: 33584203
- Sun W et al. Tetrahydropyrimidines, ZL-5015 Alleviated Lipopolysaccharide (LPS)-Induced Acute Pneumonia in Rats by Activating the NRF-2/HO-1 Pathway. Med Sci Monit 26:e924482 (2020). PubMed: 32844782
- Cornelius DC et al. NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture. PLoS One 15:e0234039 (2020). PubMed: 32555710
- Cornelius DC et al. NLRP3 inflammasome activation in platelets in response to sepsis. Physiol Rep 7:e14073 (2019). PubMed: 31054188
- He T et al. Effectiveness of Huai Qi Huang Granules on Juvenile Collagen-induced Arthritis and Its Influence on Pyroptosis Pathway in Synovial Tissue. Curr Med Sci 39:784-793 (2019). PubMed: 31612397
- Xia P et al. Pioglitazone Confers Neuroprotection Against Ischemia-Induced Pyroptosis due to its Inhibitory Effects on HMGB-1/RAGE and Rac1/ROS Pathway by Activating PPAR-?. Cell Physiol Biochem 45:2351-2368 (2018). PubMed: 29554649
- Lin ZH et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia. Front Cell Neurosci 11:391 (2017). PubMed: 29311826