Rat IL-18 ELISA Kit (ab213909)

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Overview

  • Product name

    Rat IL-18 ELISA Kit
    See all IL-18 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 1 16 126pg/ml 6 4.7%
    Sample 2 16 335pg/ml 19.43 5.8%
    Sample 3 16 592pg/ml 36.7 6.2%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 24 147pg/ml 7.94 5.4%
    Sample 2 24 392pg/ml 24.7 6.3%
    Sample 3 24 644pg/ml 46.4 7.2%

  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 1 pg/ml

  • Range

    15.6 pg/ml - 1000 pg/ml

  • Assay time

    3h 30m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    The Rat IL-18 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213909) is designed for the quantitative measurement of Rat IL-18 in cell culture supernatants, serum and plasma (EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IL-18 amount of sample captured in plate.

  • Notes

    Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.  IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat IL-18 ELISA Kit (ab213909) Standard Curve
    Rat IL-18 ELISA Kit (ab213909) Standard Curve

    Rat IL-18 ELISA Kit (ab213909) Standard Curve

Protocols

References

This product has been referenced in:

  • Cornelius DC  et al. NLRP3 inflammasome activation in platelets in response to sepsis. Physiol Rep 7:e14073 (2019). Read more (PubMed: 31054188) »
  • Lin ZH  et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia. Front Cell Neurosci 11:391 (2017). Read more (PubMed: 29311826) »
See all 2 Publications for this product

Publishing research using ab213909? Please let us know so that we can cite the reference in this datasheet.

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