Overview

  • Product name

    Rat IL-2 ELISA Kit
    See all IL-2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 3 4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 5 4.2%
  • Sample type

    Serum, Cell culture media, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.9 pg/ml
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93 89% - 100%
    Tissue Extracts 45 43% - 48%
    Cell culture media 108 99% - 113%
    Citrate Plasma 86 84% - 88%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Rat
    Does not react with: Mouse
  • Product overview

    IL-2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 protein in rat serum, plasma, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples diluted in Sample Diluent NS - 4.9 pg/mL
    Samples diluted in Sample Diluent 60BS - 12.5 pg/mL

  • Notes

    IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical monomeric polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, natural killer cell (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, thereby regulating cytokine cascades that correlate with each of the Th differentiation states. Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat IL-2 Capture Antibody 1 x 600µl
    10X Rat IL-2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Rat IL-2 Lyophilized Recombinant Protein 2 vials
    Sample Diluent 75BS 1 x 20ml
    Sample Diluent NS 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in disease

    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similarities

    Belongs to the IL-2 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • OTTHUMP00000164090
    • POIL2
    • T Cell Growth Factor
    • T-cell growth factor
    • TCGF
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab221834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of rat IL-2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

  • Example of rat IL-2 standard curve in Sample Diluent 60BS. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen (2 days) supernatant samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 6401 pg/mL in Concanavalin A treated rat spleen supernatant.

     

     

  • Interpolated concentrations of spiked IL-2 in rat serum and plasma samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Comparison of IL-2 in unstimulated and Concanavalin A stimulated rat spleen supernatants. Rat spleen was cultured for 2 days in the presence or absence of 5 µg/mL Concanavalin A. The concentrations of IL-2 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-2 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-2 concentration was determined to be 6042 pg/mL in Concanavalin A stimulated neat rat spleen supernatant and undetectable in unstimulated rat spleen supernatant and RPMI media (not shown).

Protocols

References

This product has been referenced in:

  • Alkhedaide AQ Anti-inflammatory Effect of Juniperus Procera Extract in Rats Exposed to Streptozotocin Toxicity. Antiinflamm Antiallergy Agents Med Chem 18:71-79 (2019). Read more (PubMed: 30474537) »
  • Alam MF  et al. Thymoquinone Ameliorates Doxorubicin-Induced Cardiotoxicity in Swiss Albino Mice by Modulating Oxidative Damage and Cellular Inflammation. Cardiol Res Pract 2018:1483041 (2018). Read more (PubMed: 29805796) »
See all 3 Publications for this product

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