Rat IL-3 Antibody Pair - BSA and Azide free (ab253482)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Rat
Overview
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Product name
Rat IL-3 Antibody Pair - BSA and Azide free
See all IL-3 kits -
Assay type
ELISA set -
Range
34.375 pg/ml - 2200 pg/ml -
Species reactivity
Reacts with: Rat -
Product overview
Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
The Antibody Pair can be used to quantify Rat IL-3. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277709), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Rat IL-3 Capture Antibody (unconjugated) 1 x 100µg Rat IL-3 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. -
Tissue specificity
Activated T-cells, mast cells, natural killer cells. -
Sequence similarities
Belongs to the IL-3 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
see all -
Database links
- Entrez Gene: 24495 Rat
- SwissProt: P04823 Rat
- Unigene: 10652 Rat
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab253482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Example of Rat IL-3 standard curve.
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277709), which uses the same antibody pair. Note that the reference range provided for the pair is an estimation based on the performance of the related kit.
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Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253482)To learn more about the advantages of recombinant antibodies see here.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab253482 has not yet been referenced specifically in any publications.
