• Product name

    Rat IL-4 ELISA Kit
    See all IL-4 kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    A 18 379.3pg/ml 13.5 2.5%
    B 19 532.5pg/ml 5.99 1.6%
    Sample n Mean SD CV%
    A 6 128.6pg/ml 7.6 5.9%
    B 6 532.2pg/ml 20.6 3.8%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    31.25 pg/ml - 1000 pg/ml
  • Assay time

    4h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s IL-4 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in cell culture supernatants, buffered solutions and plasma.

    A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentRations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substRate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentRation of IL-4 present in the samples.

    This kit will recognize both endogenous and recombinant rat IL-4.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-4 Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-4 Microplate (12 x 8 well strips) 1 unit 2 units
    Rat IL-4 Standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Relevance

    IL-4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL-4 encoding distinct isoforms have been reported.
  • Cellular localization

  • Alternative names

    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF-1
    • BSF1
    • IL 4
    • IL4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • Database links


Our Abpromise guarantee covers the use of ab46073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.



    Representative Standard Curve using ab46073



ab46073 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


I have the following information for you regarding the kits we discussed:

We have no data regarding normal values observed with IL-6 and IL-4 in rat but as free circulating cytokines are rather dangerous for living animal, the normal level is probably below the detection level.

Unfortunately, we have not tested the rat IL-6 ELISA on normal level in rat samples. We have only tested this ELISA using samples spiked with recombinant protein.

ab100770 and ab100772:
1) What is the normal amount of rat IL4 in serum?
We tested the normal amount of IL-4 in healthy rat serum/plasma samples to be undetectable – 2 pg/ml.

2) What is the normal amount of rat IL6 in serum?
We tested the normal amount of IL-6 in healthy rat serum/plasma samples to be below the detectable limits of this ELISA. Thus, this kit may be more suitable for diseased/treated samples; however, there are some attached tips for increasing sensitivity the customer may use - see below.

3) What is the recommended dilution range for rat serum for IL4 and IL6?
We recommend a 2-fold dilution for serum/plasma samples for use with our IL-4 and IL-6 Rat ELISA kits.

Some advice for what to do if samples are reading below detection limit and how the sensitivity of the kit can be increased:

Incubate sample overnight at 4 degrees C
Increase amount of biotinylated ab (by 1.5 fold or so – too much may increase background)
Incubate biotinylated ab overnight at 4 degrees C
Increase amount of HRP-strep (by about 1.5 fold or so – too much may increase background)
Concentrate sample (using a spin column)

Please note: it’s best to try just one of these modifications at a time. Implementing too many of these changes at once may cause high background.

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