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    rat-il-6-elisa-kit-ab234570.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantSimpleStep

Rat IL-6 ELISA Kit (ab234570)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2) Submit a question References (17)

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Other - Rat IL-6 ELISA Kit (ab234570)
  • Example of Rat IL-6 standard curve in Sample Diluent NS
  • Example of Rat IL-6 standard curve in Sample Diluent NBS
  • Interpolated concentrations of spike IL-6 in Rat serum and plasma samples
  • Interpolated concentrations of native IL-6 in Rat spleen cell culture supernatant sample
  • Rat Il-6 competitor standard curve comparison
  • Sandwich ELISA - Rat IL-6 ELISA Kit (ab234570)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 43 pg/ml
  • Range: 125 pg/ml - 8000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

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Overview

  • Product name

    Rat IL-6 ELISA Kit
    See all IL-6 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Spiked media 5 2.3%
    Inter-assay
    Sample n Mean SD CV%
    Spiked media 3 3.8%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    43 pg/ml
  • Range

    125 pg/ml - 8000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91 82% - 99%
    Serum 98 86% - 112%
    Hep Plasma 81 79% - 84%
    EDTA Plasma 94 93% - 96%
    Cit plasma 86 80% - 90%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Rat IL-6 ELISA Kit (ab234570) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-6 with 43 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat IL-6 Capture Antibody 1 x 600µl
    10X Rat IL-6 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Plate Seals 1 unit
    Rat IL-6 Lyophilized Recombinant Protein 2 vials
    Sample Diluent NBS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
    • Neurology process
    • Metabolism
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Processes
  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

    • Entrez Gene: 24498 Rat
    • SwissProt: P20607 Rat
    • Unigene: 9873 Rat

    Associated products

    • Alternative Versions

      • Rat IL-6 Antibody Pair - BSA and Azide free (ab244116)

    Images

    • Other - Rat IL-6 ELISA Kit (ab234570)
      Other - Rat IL-6 ELISA Kit (ab234570)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Rat IL-6 standard curve in Sample Diluent NS
      Example of Rat IL-6 standard curve in Sample Diluent NS

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of Rat IL-6 standard curve in Sample Diluent NBS
      Example of Rat IL-6 standard curve in Sample Diluent NBS

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spike IL-6 in Rat serum and plasma samples
      Interpolated concentrations of spike IL-6 in Rat serum and plasma samples

      The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50%, and cell culture media 50%.

    • Interpolated concentrations of native IL-6 in Rat spleen cell culture supernatant sample
      Interpolated concentrations of native IL-6 in Rat spleen cell culture supernatant sample

      The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 665 pg/mL in spleen supernatant.

    • Rat Il-6 competitor standard curve comparison
      Rat Il-6 competitor standard curve comparison

      Standard curve comparison between human FGF19 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

    • Sandwich ELISA - Rat IL-6 ELISA Kit (ab234570)
      Sandwich ELISA - Rat IL-6 ELISA Kit (ab234570)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (17)

    Publishing research using ab234570? Please let us know so that we can cite the reference in this datasheet.

    ab234570 has been referenced in 17 publications.

    • Ponomarenko AI  et al. N-docosahexaenoylethanolamine reduces neuroinflammation and cognitive impairment after mild traumatic brain injury in rats. Sci Rep 11:756 (2021). PubMed: 33436960
    • Chen S  et al. Anti-inflammatory effect of salusin-ß knockdown on LPS-activated alveolar macrophages via NF-?B inhibition and HO-1 activation. Mol Med Rep 23:N/A (2021). PubMed: 33300078
    • Qian X  et al. Therapeutic Effect of Idebenone on Rats with Vascular Dementia via the MicroRNA-216a/RSK2/NF-?B Axis. Neuropsychiatr Dis Treat 17:533-543 (2021). PubMed: 33628024
    • Zheng W  et al. Identification of miR-145 as a regulator of the cardiomyocyte inflammatory response and oxidative stress under hyperglycemia. Exp Ther Med 21:467 (2021). PubMed: 33763154
    • Wang X  et al. Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1. Med Sci Monit 27:e928480 (2021). PubMed: 33931577
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Used to measure IL-6 in rat milk samples

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Frozen maternal milk samples were defrosted on wet ice and centrifuged at 10000 rmp at 4 degrees C for 10 minutes to isolate the clear whey from the fat layer. Undiluted milk whey and blood plasma (obtained by spinning blood at 1000 g in EDTA coated tubes for 10 min at room temperature) were loaded onto the plate and processed. The kit instructions were easy to follow and we obtained results quickly.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 16 2022

    Testing IL-6 levels in rat spinal cord.

    Average Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    We tested IL-6 levels in samples of homogenized rat spinal cord. The results were alright and most of the CV values were acceptable. It was fast and easy to use.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

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