Rat IL-6 ELISA Kit (ab234570)
- Datasheet
- References
- Protocols
Overview
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Product name
Rat IL-6 ELISA Kit
See all IL-6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Spiked media 5 2.3% Inter-assay Sample n Mean SD CV% Spiked media 3 3.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
43 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 91 82% - 99% Serum 98 86% - 112% Hep Plasma 81 79% - 84% EDTA Plasma 94 93% - 96% Cit plasma 86 80% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
IL-6 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 protein in Rat serum, plasma and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Interleukin 6(IL-6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL-6 is Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL-6. IL-6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Sensitivity:
Samples diluted in Sample Diluent NS - 45 pg/mL
Samples diluted in Sample Diluent NBS - 43 pg/mL
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IL-6 Capture Antibody 1 x 600µl 10X Rat IL-6 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat IL-6 Lyophilized Recombinant Protein 2 vials Sample Diluent NBS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
Database links
- Entrez Gene: 24498 Rat
- SwissProt: P20607 Rat
- Unigene: 9873 Rat
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab234570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Rat IL-6 ELISA Kit (ab234570)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Rat IL-6 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of Rat IL-6 standard curve in Sample Diluent NBSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike IL-6 in Rat serum and plasma samplesThe concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50%, and cell culture media 50%.
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Interpolated concentrations of native IL-6 in Rat spleen cell culture supernatant sampleThe concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 665 pg/mL in spleen supernatant.
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Rat Il-6 competitor standard curve comparisonStandard curve comparison between human FGF19 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Protocols
References
ab234570 has not yet been referenced specifically in any publications.
