• Product name
    Rat IL-6 ELISA Kit (Interleukin-6)
    See all IL6 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    40.96 pg/ml - 10000 pg/ml
  • Recovery

    119 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 130.2 118% - 138%
    Plasma 122.8 109% - 138%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-6 (Interleukin-6) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat IL-6 in plasma or cell culture supernatants.

    This assay employs an antibody specific for Rat IL-6 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Due to the relatively low abundance of IL-6 in normal rat plasma, this kit may not be suitable for use with this sample type. We have not been able to detect endogenous Rat IL-6 in normal serum with ab100772, only in serum spiked with Rat IL-6.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Rat IL-6 (lyophilized) 2 vials
    IL6 Microplate (12 x 8 wells) 1 unit
    Recombinant Rat IL-6 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    N- and O-glycosylated.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating fact
    • HGF
    • HPGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6 protein
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    • Interleukin6
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100772



This product has been referenced in:
  • Mazur A  et al. Angiotensin Converting Enzyme Inhibitor Has a Protective Effect on Decompression Sickness in Rats. Front Physiol 9:64 (2018). Functional Studies ; Rat . Read more (PubMed: 29545754) »
  • Liu Z  et al. Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats. Evid Based Complement Alternat Med 2018:3674145 (2018). Read more (PubMed: 29541141) »

See all 10 Publications for this product

Customer reviews and Q&As

AbTrial test for rat lung tissue lysate samples

Inconclusive Excellent 5/5 (Ease of Use)
Tried rat lung lysate (in PBS) on kit ab100772, all the readings are either negative or below detection range. So this kit is not a good fit for tissue lysate

Abcam user community

Verified customer

Submitted Feb 09 2016

the product worked effectively.

Newman Osafo

Verified customer

Submitted Dec 17 2014

The lab adviced the following:

While serum and plasma samples maybe run undiluted, we do not usually recommend doing so. The reason is not because Assay Diluent A needs to be present in every sample but that testing undiluted serum/plasma...

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I have the following information for you regarding the kits we discussed:

We have no data regarding normal values observed with IL-6 and IL-4 in rat but as free circulating cytokines are rather dangerous for living ani...

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Thank you for your inquiry.

I heard back from the lab that you should try diluting the plasma samples because this minimizes the chances of matrix effects which can lead to inaccurate detection or non-linear responses. Matrix effects happen w...

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Perdona por la espera.
Es muy posible que el motivo de la baja absorbancia observada en las placas se deba a la perdida de actividad de alguno de los componentes del kit, debido a su degradación por haberse mantenido durante tantos meses a 4C. Read More

Gracias por mantenerte a la espera.
Después de discutir el caso con mis compañeros, hemos comentado lo siguiente:
- Ambos kits se pidieron en Junio, si no se han utilizado hasta ahora es posible que haya habido degradación de los reactivos de...

Read More

Gracias por mandarme los cuestionarios rellenos.

¿Podrías por favor confirmarme como se llevo a cabo la preparación de la solución de HRP Streptavidina?

Recientemente se han cambiado los protocolos de alg...

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Gracias por contactarnos.

Vuelvo a pediros disculpas por las molestias causadas, y siento mucho que estos kits no hayan dado el resultado esperado.

Para poder investigar este caso, evitar que vuelva a repetirse, y desde luego estar ...

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Thank you for your enquiry and your interest.

This kit is in stock and if you place the order today, we could deliver it by Friday the 28th September. The unit price is €490.00. I would advise you to read the datasheet as well as the on-...

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1-10 of 16 Abreviews or Q&A


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