Rat Interferon gamma ELISpot Pair (ab281994)
Key features and details
- Assay type: ELISPOT
- Detection method: Colorimetric
- Sample type: Suspension cells
- Reacts with: Rat
Overview
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Product name
Rat Interferon gamma ELISpot Pair
See all Interferon gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 5.3% Overall 3 7.1% Overall 3 6.8% Overall 3 17.5% Overall 3 28.4% -
Sample type
Suspension cells -
Assay type
ELISPOT -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat Interferon gamma ELISpot Antibody Pair (ab281994) is an antibody pair designed for analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level by the ELISpot method.
For the quantitative determination of the frequency of Rat Interferon gamma-producing cells.
For research use only - not intended for diagnostic use.
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Tested applications
Suitable for: ELISpotmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 10 x 96 tests Biotinylated Rat Interferon gamma detection antibody 2 vials Rat Interferon gamma Capture Antibody 2 x 0.5ml -
Research areas
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 25712 Rat
- SwissProt: P01581 Rat
- Unigene: 10795 Rat
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISpot |
Use at an assay dependent concentration.
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Notes |
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ELISpot
Use at an assay dependent concentration. |
Datasheets and documents
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Datasheet download
References (0)
ab281994 has not yet been referenced specifically in any publications.