Overview

  • Product name
    Rat KIM-1 TIM-1 ELISA Kit
    See all TIM 1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s rat KIM-1 (TIM-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat KIM-1 (TIM-1) in cell culture supernatants, tissue homogenates, serum, plasma (heparin, EDTA) and urine.

    A KIM-1 (TIM-1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat KIM-1 (TIM-1) amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve
    Typical Standard Curve

    Representaive Standard Curve using ab119597

Protocols

References

This product has been referenced in:
  • Wang Y  et al. Cinnabar-induced subchronic renal injury is associated with increased apoptosis in rats. Biomed Res Int 2015:278931 (2015). Rat . Read more (PubMed: 25629042) »

See 1 Publication for this product

Publishing research using ab119597? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

10
Votes: Highest First

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches ...

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Thank you for contacting us.


To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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Inquiry: Hi, We're planning to use the KIM-1 TIM-1 Rat ELISA kit to measure this in rat tissue samples. We were looking at the sample preparation section and wondered if PBS and homogenisation would be sufficient to disrupt kidney tissue enough to get ...

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I can confirm that PBS and homogenization will be absolutely fine and can be used flawlessly with the kit. Using tissue lysis buffer/solution is even better but PBS alone should work perfectly well.

I've been using this kit with rat urine. Is this kit pH dependent? Should we be adjusting the pH of our samples to ensure accuracy?

The pH of rat urine is close to 7.0, and there is no need to adjust the pH before use with the kit.

I found you have the Rat Kim-1 ELISA kit. Do you have the Rat Kim-1 antibody for immuohistochemistry?

Thank you for contacting us.
We have several TIM1 antibodies (TIM1 is a synonym for KIM1).
Two of these antibodies were tested in IHC with paraffin sections with human and mouse, but not rat.
If you like to try using these antibodies in ...

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As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches ...

Read More

I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Li...

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Thank you for your prompt reply. This is the information I needed. I am also looking to purchase a similar style ELISA kit for rat kidney injury molecule-1 (Rat KIM-1). Does your company sell that item?

Thank you for contacting us. It is my pleasure to help.

We do offer a KIM1 rat ELISA kit as well. This kit has a similar system containing one plate made of twelve 8 well strips. The product number is ab119597 and may be found at the follo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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