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    rat-kim-1-tim-1-elisa-kit-ab119597.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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Rat KIM-1 TIM-1 ELISA Kit (ab119597)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (6)References (5)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 2 pg/ml
    • Range: 31.2 pg/ml - 2000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

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    Overview

    • Product name

      Rat KIM-1 TIM-1 ELISA Kit
      See all TIM 1 kits
    • Detection method

      Colorimetric
    • Sample type

      Cell culture supernatant, Urine, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 2 pg/ml
    • Range

      31.2 pg/ml - 2000 pg/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Rat
    • Product overview

      Abcam’s rat KIM-1 (TIM-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat KIM-1 (TIM-1) in cell culture supernatants, tissue homogenates, serum, plasma (heparin, EDTA) and urine.

      A KIM-1 (TIM-1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat KIM-1 (TIM-1) amount of sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-rat KIM-1 antibody Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-rat KIM-1 antibody 1 x 130µl
      Lyophilized recombinant rat KIM-1 standard 2 x 10ng
      Plate Seal 4 units
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Amber Bottle 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • Non-CD
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Immunology
      • Immune System Diseases
      • Allergens
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      • Kits
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    • Function

      May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
    • Tissue specificity

      Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. TIM family.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q96D42 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • CD365
      • HAVCR
      • HAVCR 1
      • HAVcr-1
      • Havcr1
      • Hepatitis A virus cellular receptor 1
      • Kidney injury molecule 1
      • KIM 1
      • KIM-1
      • T cell immunoglobin domain and mucin domain protein 1
      • T cell immunoglobulin mucin family member 1
      • T cell immunoglobulin mucin receptor 1
      • T-cell immunoglobulin and mucin domain-containing protein 1
      • T-cell membrane protein 1
      • TIM
      • TIM-1
      • TIM1
      • TIMD 1
      • TIMD-1
      • TIMD1
      • TIMD1_HUMAN
      see all
    • Database links

      • Entrez Gene: 286934 Rat
      • SwissProt: O54947 Rat

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representaive Standard Curve using ab119597

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (5)

        Publishing research using ab119597? Please let us know so that we can cite the reference in this datasheet.

        ab119597 has been referenced in 5 publications.

        • Almeer RS  et al. Royal jelly attenuates cadmium-induced nephrotoxicity in male mice. Sci Rep 9:5825 (2019). PubMed: 30967588
        • Joshi MD  et al. Evaluation of Fetal and Maternal Vancomycin-Induced Kidney Injury during Pregnancy in a Rat Model. Antimicrob Agents Chemother 63:N/A (2019). PubMed: 31332061
        • Perales-Quintana MM  et al. Metabolomic and biochemical characterization of a new model of the transition of acute kidney injury to chronic kidney disease induced by folic acid. PeerJ 7:e7113 (2019). PubMed: 31275747
        • Lin Z  et al. Fish oils protects against cecal ligation and puncture-induced septic acute kidney injury via the regulation of inflammation, oxidative stress and apoptosis. Int J Mol Med 44:1771-1780 (2019). PubMed: 31545434
        • Wang Y  et al. Cinnabar-induced subchronic renal injury is associated with increased apoptosis in rats. Biomed Res Int 2015:278931 (2015). Rat . PubMed: 25629042

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        1-6 of 6 Abreviews or Q&A

        Question

        As I’m not familiar with these items, I tried to find the ab codes through your site.
        For the items I found a relative code I have update the list.
        For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
        So please check the list again and advice.
        https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
        https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
        https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
        https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
        Porcine Noradrenalin ELISA kit 96 Tests (No product match)
        Porcine Adrenaline ELISA kit 96 Tests (No product match)
        https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
        Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
        https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
        Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
        Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
        https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
        Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
        https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

        Read More

        Abcam community

        Verified customer

        Asked on Jun 25 2012

        Answer

        Thank you for contacting us.


        To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the details soon.

        The other kits ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed.

        I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

        Use our products? Submit an Abreview. Earn rewards!
        https://www.abcam.com/abreviews

        Read More

        Abcam Scientific Support

        Answered on Jun 25 2012

        Question

        Inquiry: Hi, We're planning to use the KIM-1 TIM-1 Rat ELISA kit to measure this in rat tissue samples. We were looking at the sample preparation section and wondered if PBS and homogenisation would be sufficient to disrupt kidney tissue enough to get these molecules into suspension?? Look forward to hearing back from you soon. Kind regards

        Read More

        Abcam community

        Verified customer

        Asked on Jun 19 2014

        Answer

        I can confirm that PBS and homogenization will be absolutely fine and can be used flawlessly with the kit. Using tissue lysis buffer/solution is even better but PBS alone should work perfectly well.

        Read More

        Padamjeet Singh

        Abcam Scientific Support

        Answered on Jun 19 2014

        Question

        I've been using this kit with rat urine. Is this kit pH dependent? Should we be adjusting the pH of our samples to ensure accuracy?

        Read More

        Abcam community

        Verified customer

        Asked on Apr 08 2013

        Answer

        The pH of rat urine is close to 7.0, and there is no need to adjust the pH before use with the kit.

        Read More

        Abcam Scientific Support

        Answered on Apr 08 2013

        Question

        I found you have the Rat Kim-1 ELISA kit. Do you have the Rat Kim-1 antibody for immuohistochemistry?

        Read More

        Abcam community

        Verified customer

        Asked on Jul 03 2012

        Answer

        Thank you for contacting us.
        We have several TIM1 antibodies (TIM1 is a synonym for KIM1).
        Two of these antibodies were tested in IHC with paraffin sections with human and mouse, but not rat.
        If you like to try using these antibodies in rat, you could use our testing discount program for untested species.
        For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
        The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
        This program applies to ELISA kits and other kits we offer.
        Please let me know if you would be interested in receiving a discount code.
        I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
        Use our products? Submit an Abreview. Earn rewards!
        https://www.abcam.com/abreviews

        Read More

        Abcam Scientific Support

        Answered on Jul 03 2012

        Question

        As I’m not familiar with these items, I tried to find the ab codes through your site.
        For the items I found a relative code I have update the list.
        For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
        So please check the list again and advice.
        https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
        https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
        https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
        https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
        Porcine Noradrenalin ELISA kit 96 Tests (No product match)
        Porcine Adrenaline ELISA kit 96 Tests (No product match)
        https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
        Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
        https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
        Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
        Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
        https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
        Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
        https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

        Read More

        Abcam community

        Verified customer

        Asked on Jun 26 2012

        Answer

        I have received information regarding

        ab119589 Cystatin C Human ELISA Kit
        ab119590 Cystatin C Mouse ELISA Kit
        ab119596 KIM1 Mouse ELISA Kit
        ab119597 KIM1 Rat ELISA Kit
        ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
        ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit

        these has not been tested with Swine or pig samples so we are unable to confirm the species cross reactivity. It mean only ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed. Would you like to have a quote for these antibodies.

        I hope this info will be helpful. Should you require more information please do not hesitate to contact me.

        Read More

        Abcam Scientific Support

        Answered on Jun 26 2012

        Question

        Thank you for your prompt reply. This is the information I needed. I am also looking to purchase a similar style ELISA kit for rat kidney injury molecule-1 (Rat KIM-1). Does your company sell that item?

        Read More

        Abcam community

        Verified customer

        Asked on Jun 06 2012

        Answer

        Thank you for contacting us. It is my pleasure to help.

        We do offer a KIM1 rat ELISA kit as well. This kit has a similar system containing one plate made of twelve 8 well strips. The product number is ab119597 and may be found at the following link:

        https://www.abcam.com/KIM1-Rat-ELISA-Kit-ab119597.html

        I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

        Use our products? Submit an Abreview. Earn rewards!
        https://www.abcam.com/abreviews

        Read More

        Abcam Scientific Support

        Answered on Jun 06 2012

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

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