Rat Laminin ELISA Kit (ab119573)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Laminin ELISA Kit
See all Laminin beta 2 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s rat Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat Laminin in cell culture supernatants, serum, cell lysates and plasma (heparin, EDTA).
A Laminin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards (form rat sarcoma basement membrane) and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat Laminin amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat Laminin Antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-rat Laminin antibody 1 x 100µl Lyophilized rat Laminin standard 2 vials Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
- Information by UniProt
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Alternative names
- Lamb2
- LAMB2_HUMAN
- Laminin B1s chain
see all -
Database links
- Entrez Gene: 25473 Rat
- SwissProt: P15800 Rat
- Unigene: 774 Rat
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab119573 has not yet been referenced specifically in any publications.