Overview

  • Product name

    Rat Leptin ELISA Kit
    See all Leptin kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 30 pg/ml
  • Range

    10.97 pg/ml - 8000 pg/ml
  • Recovery

    84 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.43 68% - 89%
    Serum 92.32 83% - 102%
    Plasma 84.25 75% - 94%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s Leptin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Leptin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Rat Leptin measured in supernatanses showing quantity (ng) per mL of tested sample

  • Rat Leptin measured in biofluids showing quantity (ng) per mL of tested sample

  • Standard curve: mean of duplicates (+/-SD) with background readings subtracted

Protocols

References

This product has been referenced in:

  • Lin YJ  et al. Maternal high-fat diet sex-specifically alters placental morphology and transcriptome in rats: Assessment by next-generation sequencing. Placenta 78:44-53 (2019). Read more (PubMed: 30955710) »
  • Yuan F  et al. Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel Expression. Front Physiol 9:249 (2018). Read more (PubMed: 29636698) »
See all 11 Publications for this product

Customer reviews and Q&As

Filter by Ratings

Abreview for Leptin Rat ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Rat Serum (Blood)
Specification: Blood
Amount of Sample: 50 µg
Type of Kit Used: ELISA
Positive control: rat serum (diabetic)
Did you use the Abcam Kit protocol?: Yes

Dr. Asmat Salim

Verified customer

Submitted May 07 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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