Overview

  • Product name

    Rat Leptin ELISA Kit
    See all Leptin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Rat serum 5 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Rat serum 3 3.7%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    24 pg/ml
  • Range

    47 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 106 100% - 106%
    Cell culture media 113 109% - 116%
    Heparin Plasma 116 108% - 120%
    Citrate Plasma 111 109% - 113%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Cow
  • Product overview

    Rat Leptin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Leptin protein in rat serum, plasma and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans, mice, and rat can cause obesity. Circulating levels of Leptin are regulated by food intake, insulin levels and pregnancy status. Rat leptin has 96% and 85% protein sequence identity to mouse and human leptin, respectively

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab229891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between Rat Leptin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • The concentrations of Leptin were measured in duplicates, interpolated from the Leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Target concentration was determined to be 1889 pg/mL in serum, 1326 pg/mL in plasma (heparin) and 1026 pg/mL in plasma (citrate).

  • The concentrations of Leptin were measured in duplicates, interpolated from the Leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The Leptin standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab229891 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229891.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up