Overview

  • Product name

    Rat Lipocalin-2 ELISA Kit
    See all Lipocalin-2 / NGAL kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    U1 8 = 3%
    U2 8 = 3%
    P1 8 = 3%
    P2 8 = 4%
    Inter-assay
    Sample n Mean SD CV%
    Urine 6 = 10%
    Plasma 5 = 12%
  • Sample type

    Urine, Serum, Plasma, Tissue Extracts, Tissue Culture Media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.5 pg/ml
  • Range

    4 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine = 106 61pg/ml - 350pg/ml
    Plasma = 104 54pg/ml - 349pg/ml

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Abcam’s Rat Lipocalin-2 ELISA Kit (ab207925) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Neutrophil Gelatinase-Associated Lipocalin levels in urine, plasma or serum, tissue extracts or culture media samples.


    The assay is a sandwich ELISA performed in microwells coated with a mouse monoclonal antibody against Rat NGAL. Bound NGAL is detected with another mouse monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate.


    Please be aware this kit is supplied with a pre-coated 96-well plate that does not break into strips. 

  • Notes

    NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are secreted proteins characterized by their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, to bind to specific cell-surface receptors, and to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGAL’s ability to bind bacterial siderophores. In the rat, NGAL was first named 25 kDa α2-microglobulin-related protein, and later neurelated lipocalin (NRL) because of its expression in mammary tumors initiated by the neu (HER2/c-erbB-2) oncogene. In the mouse, the homologous protein is known as oncogene protein 24p3, 24-kDa superinducible protein (SIP24) or uterocalin.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    25x Wash Buffer Solution 1 x 40ml
    5x Sample Diluent Buffer 1 x 50ml
    Biotinylated Rat-NGAL Antibody 1 x 12ml
    HRP-Streptavidin Solution 1 x 12ml
    Microwell plate, 96 precoated wells 1 x 96 tests
    Rat NGAL Standard (0 pg/mL) 1 x 1ml
    Rat NGAL Standard (10 pg/mL) 1 x 1ml
    Rat NGAL Standard (100 pg/mL) 1 x 1ml
    Rat NGAL Standard (20 pg/mL) 1 x 1ml
    Rat NGAL Standard (200 pg/mL) 1 x 1ml
    Rat NGAL Standard (4 pg/mL) 1 x 1ml
    Rat NGAL Standard (40 pg/mL) 1 x 1ml
    Rat NGAL Standard (400 pg/mL) 1 x 1ml
    Stop Solution 1 x 12ml
    TMB Substrate Solution 1 x 12ml
  • Research areas

  • Function

    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificity

    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • Alternative names

    • 24p3
    • 25 kDa alpha 2 microglobulin related subunit of MMP9
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
    • Alpha 2 microglobulin related protein
    • HGNC:6526
    • HNL
    • Lcn 2
    • Lcn2
    • Lipocalin-2
    • Migration stimulating factor inhibitor
    • MSFI
    • Neutrophil gelatinase associated lipocalin
    • Neutrophil gelatinase associated lipocalin precursor
    • Neutrophil gelatinase-associated lipocalin
    • NGAL
    • NGAL_HUMAN
    • Oncogene 24p3
    • Oncogenic lipocalin 24p3
    • p25
    • Siderocalin
    • siderocalin LCN2
    • SV40 induced 24P3 protein
    • Uterocalin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab207925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Abcam’s Rat Lipocalin-2 ELISA Kit (ab207925) Calibration Curve

Protocols

References

This product has been referenced in:

  • Popovic D  et al. Protective effects of anthocyanins from bilberry extract in rats exposed to nephrotoxic effects of carbon tetrachloride. Chem Biol Interact 304:61-72 (2019). Read more (PubMed: 30825423) »
See 1 Publication for this product

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