Key features and details
- Sensitivity: 0.5 pg/ml
- Range: 4 pg/ml - 400 pg/ml
- Sample type: Plasma, Serum, Tissue Culture Media, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat Lipocalin-2 ELISA Kit
See all Lipocalin-2 / NGAL kits
Intra-assay Sample n Mean SD CV% U1 8 = 3% U2 8 = 3% P1 8 = 3% P2 8 = 4% Inter-assay Sample n Mean SD CV% Urine 6 = 10% Plasma 5 = 12%
Sample typeUrine, Serum, Plasma, Tissue Extracts, Tissue Culture Media
Assay typeSandwich (quantitative)
Sensitivity= 0.5 pg/ml
Range4 pg/ml - 400 pg/ml
Sample specific recovery Sample type Average % Range Urine = 106 61pg/ml - 350pg/ml Plasma = 104 54pg/ml - 349pg/ml
Assay time4h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rat
Abcam’s Rat Lipocalin-2 ELISA Kit (ab207925) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Neutrophil Gelatinase-Associated Lipocalin levels in urine, plasma or serum, tissue extracts or culture media samples.
The assay is a sandwich ELISA performed in microwells coated with a mouse monoclonal antibody against Rat NGAL. Bound NGAL is detected with another mouse monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate.
Please be aware this kit is supplied with a pre-coated 96-well plate that does not break into strips.
NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are secreted proteins characterized by their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, to bind to specific cell-surface receptors, and to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGAL’s ability to bind bacterial siderophores. In the rat, NGAL was first named 25 kDa α2-microglobulin-related protein, and later neurelated lipocalin (NRL) because of its expression in mammary tumors initiated by the neu (HER2/c-erbB-2) oncogene. In the mouse, the homologous protein is known as oncogene protein 24p3, 24-kDa superinducible protein (SIP24) or uterocalin.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 25x Wash Buffer Solution 1 x 40ml 5x Sample Diluent Buffer 1 x 50ml Biotinylated Rat-NGAL Antibody 1 x 12ml HRP-Streptavidin Solution 1 x 12ml Microwell plate, 96 precoated wells 1 x 96 tests Rat NGAL Standard (0 pg/mL) 1 x 1ml Rat NGAL Standard (10 pg/mL) 1 x 1ml Rat NGAL Standard (100 pg/mL) 1 x 1ml Rat NGAL Standard (20 pg/mL) 1 x 1ml Rat NGAL Standard (200 pg/mL) 1 x 1ml Rat NGAL Standard (4 pg/mL) 1 x 1ml Rat NGAL Standard (40 pg/mL) 1 x 1ml Rat NGAL Standard (400 pg/mL) 1 x 1ml Stop Solution 1 x 12ml TMB Substrate Solution 1 x 12ml
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Information by UniProt
- 25 kDa alpha 2 microglobulin related subunit of MMP9
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
ab207925 has been referenced in 3 publications.
- Jackson EK et al. Long-Term Dipeptidyl Peptidase 4 Inhibition Worsens Hypertension and Renal and Cardiac Abnormalities in Obese Spontaneously Hypertensive Heart Failure Rats. J Am Heart Assoc 10:e020088 (2021). PubMed: 33682436
- Popovic D et al. Protective effects of anthocyanins from bilberry extract in rats exposed to nephrotoxic effects of carbon tetrachloride. Chem Biol Interact 304:61-72 (2019). PubMed: 30825423
- Popovic D et al. Anthocyanins Protect Hepatocytes against CCl4-Induced Acute Liver Injury in Rats by Inhibiting Pro-inflammatory mediators, Polyamine Catabolism, Lipocalin-2, and Excessive Proliferation of Kupffer Cells. Antioxidants (Basel) 8:N/A (2019). PubMed: 31590249