Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.9 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Product nameRat Lipocalin-2 ELISA Kit
See all Lipocalin-2 / NGAL kits
Intra-assay Sample n Mean SD CV% plasma 8 7% Inter-assay Sample n Mean SD CV% plasma 3 6.8%
Sample typeCell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range7.81 pg/ml - 500 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 101% - 111% Urine 102 99% - 107% Serum 92 91% - 94% Cell culture media 90 87% - 93% Hep Plasma 101 98% - 103% EDTA Plasma 97 94% - 101% Cit plasma 91 88% - 93%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Rat
Rat Lipocalin-2 ELISA Kit (ab239422) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lipocalin-2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Lipocalin-2 with 1.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Rat Lipocalin-2 Capture Antibody 1 x 600µl 10X Rat Lipocalin-2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat Lipocalin-2 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Information by UniProt
- 25 kDa alpha 2 microglobulin related subunit of MMP9
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The Lipocalin-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:500, plasma (EDTA) 1:800, plasma (heparin) 1: 5,000 and plasma (citrate) 1: 13,333. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 293 ng/mL in serum, 290 ng/mL in plasma (EDTA), 560 ng/mL in plasma (heparin) and 4,326 ng/mL in plasma (citrate).
The concentrations of Lipocalin-2 were measured in duplicates, interpolated from the Lipocalin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:10,000 and lung cell culture supernatant 1: 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lipocalin-2 concentration was determined to be 2,184 ng/mL in urine and 297 ng/mL in lung supernatant.
To learn more about the advantages of recombinant antibodies see here.
ab239422 has not yet been referenced specifically in any publications.