Rat Lipocalin-2 ELISA Kit (NGAL) (ab119602)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Lipocalin-2 ELISA Kit (NGAL)
See all Lipocalin-2 / NGAL kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human, Pig -
Product overview
Abcam’s rat Lipocalin-2 (NGAL) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat Lipocalin-2 in cell culture supernatants, serum plasma (heparin) and urine.
A Lipocalin-2 specific mouse monoclonal antibody for Lipocalin-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat Lipocalin-2 amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-rat Lipocalin-2 antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-rat Lipocalin-2 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant rat Lipocalin-2 standard 2 x 10ng 2 x 10ng Plate Seal 4 units 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Alternative names
- 24p3
- 25 kDa alpha 2 microglobulin related subunit of MMP9
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
see all -
Database links
- Entrez Gene: 3934 Human
- Entrez Gene: 16819 Mouse
- Entrez Gene: 170496 Rat
- Omim: 600181 Human
- SwissProt: P80188 Human
- SwissProt: P11672 Mouse
- SwissProt: P30152 Rat
- Unigene: 204238 Human
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab119602 has been referenced in 11 publications.
- Vogelaar PC et al. Towards prevention of ischemia-reperfusion kidney injury: Pre-clinical evaluation of 6-chromanol derivatives and the lead compound SUL-138✰. Eur J Pharm Sci 168:106033 (2022). PubMed: 34610451
- Li T et al. Ulinastatin Improves Renal Microcirculation by Protecting Endothelial Cells and Inhibiting Autophagy in a Septic Rat Model. Kidney Blood Press Res 47:256-269 (2022). PubMed: 35016182
- Spreadborough PJ et al. Tourniquet use following blast-associated complex lower limb injury and traumatic amputation promotes end organ dysfunction and amplified heterotopic ossification formation. J Orthop Surg Res 17:422 (2022). PubMed: 36123728
- Wei L et al. Lipocalin-2 Regulates Hippocampal Microglial Activation in Poststroke Depression. Front Aging Neurosci 13:798335 (2021). PubMed: 34966272
- Moser MAJ et al. Protection of the transplant kidney during cold perfusion with doxycycline: proteomic analysis in a rat model. Proteome Sci 18:3 (2020). PubMed: 32336955