• Product name
    Rat MCP1 ELISA Kit (CCL2)
    See all MCP1 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    24.67 pg/ml - 18000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.4 86% - 105%
    Serum 95.39 84% - 103%
    Plasma 92.44 83% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s MCP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat MCP1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat MCP1 coated on a 96-well plate. Standards and samples are pipette into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab100777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100777

  • Representative standard curve using ab100777



ab100777 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product was not supplied in full and for the inconvenience this has caused. As re...

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I am sorry to hear that this kit was not supplied to you in full.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compe...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I h...

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Thank you for your enquiry.

I have addressed your questions below.
1. Firstly I need to use the kit to detect urinary MCP-1, so which Diluent should I use: Diluent A or B?

Assay Diluent A is optimized specifically for only se...

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Thank you for contacting us.

Although we have not tested and validated this ELISA kit with urine samples, based on previous testing with our ELISA kits the Rat MCP1 ELISA is very likely to work for urine.

Some general guidelines on ...

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Thank you for your enquiry. I apologize for the delay in replying.

The two kits use the same antibodies but a few other reagents in each kit differ, to optimize the assay for different sample types. Product ab100777 is best for plasma, serum...

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