Rat monoclonal [187.1] Anti-Mouse kappa light chain (HRP) (ab99617)

Overview

  • Product name
    Rat monoclonal [187.1] Anti-Mouse kappa light chain (HRP)
    See all Kappa light chain secondary antibodies
  • Host species
    Rat
  • Target species
    Mouse
  • Tested applications
    Suitable for: ELISA, ICC/IF, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Kappa light chain.

  • Conjugation
    HRP

Properties

Applications

Our Abpromise guarantee covers the use of ab99617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent concentration.

Images

  • Lane 1: Mouse IgG1 Kappa loaded at 0.2ug/well
    Lane 2: Mouse IgG3 Lambda loaded at 0.2ug/well
    ab99617 diluted to 1:2,000

References

This product has been referenced in:
  • Mason DM  et al. High-throughput antibody engineering in mammalian cells by CRISPR/Cas9-mediated homology-directed mutagenesis. Nucleic Acids Res 46:7436-7449 (2018). Read more (PubMed: 29931269) »
  • Jung J  et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. Elife 6:N/A (2017). Human . Read more (PubMed: 28195531) »
See all 4 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer



The two antibodies are essentially the same except for their clone numbers. The rest of the specifications are the same.

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Answer

Thank you for your reply.

I have had a look at the antibodies we have available form our catalogue and have come up with the following for you to consider:

against kappa light chain:

Rat monoclonal ab11662

Rat monoclonal ab99614

Rabbit polyclonal ab103827

against kappa light chain, HRP conjugated:

Goat polyclonal ab46527

Rat monoclonal ab99617

against lambda light chain:

Rat monoclonal ab99622

against lambda light chain HRP conjugated

Rat monoclonal ab99625

I hope this information has been of help. If you have any further questions, please do not hesitate to contact me again.

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Answer

Thank you for contacting us.

That is correct. This has been tested to recognize the kappa chain in each of those isotypes.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us.


I have spoken to the lab about your question. Ab99617 has been tested by ELISA on a panel of Mouse isotypes to ensure reactivity with the kappa light chain of IgM, IgA, and IgG subclasses.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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