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    rat-monoclonal-23g3-mouse-ige-epsilon-chain-hrp-ab99574.pdf

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Immunology Immunoglobulins Heavy Chain IgE
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Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (HRP) (ab99574)

  • Datasheet
Submit a review Q&A (1)References (4)

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Abpromise

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Find out more.

Key features and details

  • Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (HRP)
  • Conjugation: HRP
  • Host species: Rat
  • Isotype: IgG1
  • Suitable for: ELISA, ICC/IF

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Overview

  • Product name

    Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (HRP)
    See all IgE secondary antibodies
  • Host species

    Rat
  • Target species

    Mouse
  • Specificity

    ab99574 reacts with the epsilon heavy chain of BALB/c Mouse IgE.
  • Tested applications

    Suitable for: ELISA, ICC/IFmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol, 50% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    23G3
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgE
    • Secondary antibodies
    • anti-Mouse
    • IgE
    • Enzyme
    • HRP

Associated products

  • Alternative Versions

    • Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (ab99571)
    • Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (FITC) (ab99572)
    • Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (Alkaline Phosphatase) (ab99573)
    • Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (Biotin) (ab99585)
    • Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain - Low endotoxin, Azide free (ab99587)
    • Rat monoclonal [23G3] Anti-Mouse IgE epsilon chain (PE) (ab99588)
  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

Our Abpromise guarantee covers the use of ab99574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
ICC/IF Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab99574? Please let us know so that we can cite the reference in this datasheet.

    ab99574 has been referenced in 4 publications.

    • Wang X  et al. Liposomes with cyclic RGD peptide motif triggers acute immune response in mice. J Control Release 293:201-214 (2019). PubMed: 30527753
    • Xie Z & Yin J Chinese Birch Pollen Allergy and Immunotherapy in Mice. Inflammation N/A:N/A (2019). PubMed: 30715691
    • Kong R & Yin J Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma. Am J Transl Res 11:280-289 (2019). PubMed: 30787986
    • Xu B  et al. Anti-inflammatory effects of dihydromyricetin in a mouse model of asthma. Mol Med Rep 15:3674-3680 (2017). PubMed: 28393183

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

    I have primary antibody produced from hybridoma Ig E am not sure if it can react to your secondary antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 11 2013

    Answer

    Yes, the Abcam antibody ab99574, anti-mouse IgE, will be able to detect your antibody, which is mouse isotype IgE.

    Read More

    Abcam Scientific Support

    Answered on Mar 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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