Rat monoclonal [H139-52.1] Anti-Mouse kappa light chain (HRP) (ab99632)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab99632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ELISA 1/1000 - 1/2000.
WB Use at an assay dependent concentration.

Images

  • Comparison of an anti-mouse secondary antibody recognizing the H&L chain versus (ab97023) versus a mouse light chain specific secondary antibody (ab99632)


    Left blot: AbExcel Goat polyclonal Secondary Antibody to Mouse IgG – H&L (HRP) (ab97023), 1/50,000

    Lane 1: 0.5 µg mouse monoclonal was loaded
    Lane 2: 0.25 µg mouse monoclonal was loaded
    Lane 3: 0.125 µg mouse monoclonal was loaded

    Right blot: Rat monoclonal Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632),1/10,000

    Lane 1: 2 µg mouse monoclonal was loaded
    Lane 2: 1 µg mouse monoclonal was loaded
    Lane 3: 0.1 µg mouse monoclonal was loaded

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632) at 1/2000 dilution
    Lanes 3 - 4 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632) at 1/10000 dilution
    Lanes 5 - 6 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632) at 1/20000 dilution

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Lane 1: Mouse IgG1 Kappa loaded at 0.2ug/well
    Lane 2: Mouse IgG3 Lambda loaded at 0.2ug/well
    ab99632 diluted to 1:2,000

References

This product has been referenced in:
  • Kang W  et al. miR-375 is involved in Hippo pathway by targeting YAP1/TEAD4-CTGF axis in gastric carcinogenesis. Cell Death Dis 9:92 (2018). WB, IP . Read more (PubMed: 29367737) »
  • Hurwitz SN  et al. CD63 Regulates Epstein-Barr Virus LMP1 Exosomal Packaging, Enhancement of Vesicle Production, and Noncanonical NF-?B Signaling. J Virol 91:N/A (2017). WB . Read more (PubMed: 27974566) »
See all 4 Publications for this product

Customer reviews and Q&As

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Application
Western blot
THIS IS A GOOD ANTIBODY THAT CAN ELIMINATE HEAVY CHAIN BACKGROUND FOR IP ASSAY. PLEASE REFER THE ATTACHED DATA IF YOU WOULD LIKE TO USE IT.

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Verified customer

Submitted Oct 18 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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